Fluidic connector assembly for quick connect/disconnect

ABSTRACT

A fluidic connection assembly and methods for quickly connecting or disconnecting a tube to a port by hand and without the use of tools. A body is adapted to receive a tube therethrough, and may have at least two sides which are hinged. Each of the hinged sides has corresponding latching portions or projections located near a lower end of the body. These projections are adapted to fit into a port or other fitting and be securely held in place. The assembly may include a tube extending through a body and through a spring located between the end of the body and the end of the tube, whereby the spring exerts a force directly or indirectly against the end of the tube and against the body, thus holding the tubing securely and sealingly engaged in the port when the assembly is connected. The body may further comprise an additional body or an adapter, and/or a cap and latch. A second spring may be used to push a projecting member into a groove or notch of an adapter when an end of the adapter is inserted into one end of the latch or the body. The fluidic connection assembly is useful in analytical instrument systems, such as for in vitro applications and/or in high pressure applications, among other things, and may be used in methods for connecting, or disconnecting, tubing or a fluidic connection assembly from a port or other fitting or connection.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a Continuation of U.S. application Ser. No.15/617,792, filed Jun. 8, 2017 which is incorporated by reference as iffully set forth herein, and which claims the benefit of and priority toU.S. Provisional Patent Application Ser. No. 62/349,569, filed Jun. 13,2016, which is incorporated by reference as if fully set forth herein,U.S. application Ser. No. 15/617,792, filed Jun. 8, 2017 which is acontinuation-in-part and claims the benefit of and priority to U.S.Non-provisional patent application Ser. No. 14/922,041, filed Oct. 23,2015, which is incorporated by reference as if fully forth herein, whichin turn claims the benefit of and priority to U.S. Provisional PatentApplication Ser. No. 62/067,739, filed Oct. 23, 2014, U.S. ProvisionalPatent Application Ser. No. 62/127,276, filed Mar. 2, 2015, and U.S.Provisional Patent Application Ser. No. 62/168,491, filed May 29, 2015,each of which is hereby incorporated by reference as if fully set forthherein.

FIELD OF THE INVENTION

This invention relates generally to fluidic connections and fittingassemblies, and more specifically to fluidic connections and fittingswhich are easy to use, provide reliable sealing connections, and can beused without additional tools or equipment, and more particularly tofluidic connections and fitting assemblies for making fluidicconnections or disconnecting fluidic connections quickly, such as may beuseful in analytical instrument systems.

BACKGROUND OF THE INVENTION

There are numerous types of conventional analytical instrument (AI)systems in use and commercially available. Conventional AI systemsinclude systems for Liquid chromatography (LC), ion chromatography (IC),and gas chromatography (GC). In addition, AI systems includehigh-pressure liquid chromatography, ultra-high pressure liquidchromatography, mass spectrometry systems, micro-flow chromatographysystems, nanoflow and nano-scale chromatography systems, capillaryelectrophoresis systems, reverse-gradient chromatography systems, andsystems which include or combine one or more of the foregoing. Althoughthe following background discussion focuses on liquid chromatographysystems and applications, those skilled in the art that the referencesto LC systems and applications are exemplary only and for theconvenience of the reader, and are not limiting in any fashion.

LC systems provide well-known techniques for separating the constituentelements in a given sample. In a conventional LC system, a liquidsolvent (referred to as the “mobile phase”) is introduced from areservoir and is pumped through the LC system. The mobile phase exitsthe pump under pressure. The mobile phase then travels via tubing to asample injection valve. As the name suggests, the sample injection valveallows an operator to inject a sample into the LC system, where thesample will be carried along with the mobile phase.

In a conventional LC system, the sample and mobile phase pass throughone or more filters and often a guard column before coming to thecolumn. A typical column usually consists of a piece of tubing which hasbeen packed with a “packing” material. The “packing” consists of theparticulate material “packed” inside the column. It usually consists ofsilica- or polymer-based particles, which are often chemically bondedwith a chemical functionality. When the sample is carried through thecolumn (along with the mobile phase), the various components in thesample migrate through the packing within the column at different rates(i.e., there is differential migration of the solutes). In other words,the various components in a sample will move through the column atdifferent rates. Because of the different rates of movement, thecomponents gradually separate as they move through the column.Differential migration is affected by factors such as the composition ofthe mobile phase, the composition of the stationary phase (i.e., thematerial with which the column is “packed”), and the temperature atwhich the separation takes place. Thus, such factors will influence theseparation of the sample's various components.

Once the sample (with its components now separated) leaves the column,it flows with the mobile phase past a detector. The detector detects thepresence of specific molecules or compounds. Two general types ofdetectors are used in LC applications. One type measures a change insome overall physical property of the mobile phase and the sample (suchas their refractive index). The other type measures only some propertyof the sample (such as the absorption of ultraviolet radiation). Inessence, a typical detector in a LC system can measure and provide anoutput in terms of mass per unit of volume (such as grams permilliliter) or mass per unit of time (such as grams per second) of thesample's components. From such an output signal, a “chromatogram” can beprovided; the chromatogram can then be used by an operator to determinethe chemical components present in the sample. Additionally, LC systemsmay utilize mass spectrometric detection for identification andquantification of the sample, either in addition to, or as analternative to, the conventional detectors described previously. Ionchromatography relies on the detection of ions in solution, so mostmetallic materials in the flow path can create interference in thedetection scheme, as they create background ions.

In addition to the above components, a LC system will often includefilters, check valves, a guard column, or the like in order to preventcontamination of the sample or damage to the LC system. For example, aninlet solvent filter may be used to filter out particles from thesolvent (or mobile phase) before it reaches the pump. A guard column isoften placed before the analytical or preparative column; i.e., theprimary column. The purpose of such a guard column is to “guard” theprimary column by absorbing unwanted sample components that mightotherwise bind irreversibly to the analytical or preparative column.

In practice, various components in an LC system may be connected by anoperator to perform a given task. For example, an operator will selectan appropriate mobile phase and column, and then connect a supply of theselected mobile phase and a selected column to the LC system beforeoperation. In order to be suitable for LC applications, each connectionmust be able to withstand the typical operating pressures of the LCsystem. If the connection is too weak, it may leak. Because the types ofsolvents that are sometimes used as the mobile phase are often toxic andbecause it is often expensive to obtain and/or prepare many samples foruse, any such connection failure is a serious concern.

It is fairly common for an operator to disconnect a column (or othercomponent) from a LC system and then connect a different column (orother component) in its place after one test has finished and before thenext begins. Given the importance of leak-proof connections in LCapplications, the operator must take time to be sure the connection issufficient. Replacing a column (or other component) may occur severaltimes in a day. Moreover, the time involved in disconnecting and thenconnecting a column (or other component) is unproductive because the LCsystem is not in use and the operator is engaged in plumbing the systeminstead of preparing samples or other more productive activities. Hence,the replacement of a column (or other component) in a conventional LCsystem can involve a great deal of wasted time and inefficiencies.

Given concerns about the need for leak-free connections, conventionalconnections have been made with stainless steel tubing and stainlesssteel end fittings. More recently, however, it has been realized thatthe use of stainless steel components in a LC system have potentialdrawbacks in situations involving biological samples, and cannot beroutinely used for ion chromatography. For example, the components in asample may attach themselves to the wall of stainless steel tubing. Thispresents problems because the detector's measurements (and thus thechromatogram) of a given sample may not accurately reflect the sample ifsome of the sample's components or ions remain in the tubing and do notpass the detector. Perhaps of even greater concern, however, is the factthat ions from the stainless steel tubing may detach from the tubing andflow past the detector, thus leading to potentially erroneous results.Hence, there is a need for “biocompatible” or “metal-free” connectionsthrough the use of a material that is chemically inert with respect tosuch “biological” samples and the mobile phase used with such samples,so that ions will not be released by the tubing and thus contaminate thesample.

In many applications using selector/injector valves to direct fluidflows, and in particular in liquid chromatography, the volume of fluidsis small. This is particularly true when liquid chromatography is beingused as an analytical method as opposed to a preparative method. Suchmethods often use capillary columns and are generally referred to ascapillary chromatography. In capillary chromatography, it is oftendesired to minimize the internal volume of the selector or injectorvalve. One reason for this is that a valve having a large volume willcontain a relatively large volume of liquid, and when a sample isinjected into the valve the sample will be diluted, decreasing theresolution and sensitivity of the analytical method.

Micro-fluidic analytical processes also involve small sample sizes. Asused herein, sample volumes considered to involve micro-fluidictechniques can range from as low as volumes of only several picolitersor so, up to volumes of several milliliters or so, whereas moretraditional LC techniques, for example, historically often involvedsamples of about one microliter to about 100 milliliters in volume.Thus, the micro-fluidic techniques described herein involve volumes oneor more orders of magnitude smaller in size than traditional LCtechniques. Micro-fluidic techniques can also be expressed as thoseinvolving fluid flow rates of about 0.5 ml/minute or less.

As noted, liquid chromatography (as well as other analytical) systemstypically include several components. For example, such a system mayinclude a pump; an injection valve or autosampler for injecting theanalyte; a precolumn filter to remove particulate matter in the analytesolution that might clog the column; a packed bed to retain irreversiblyadsorbed chemical material; the LC column itself; and a detector thatanalyzes the carrier fluid as it leaves the column. Ion chromatographymay also utilize a suppressor column to facilitate detection dynamicrange. These various components may typically be connected by aminiature fluid conduit, or tubing, such as metallic or polymeric tubing(for ion chromatography), usually having an internal diameter of 0.003to 0.040 inch.

All of these various components and lengths of tubing are typicallyinterconnected by threaded fittings. Fittings for connecting various LCsystem components and lengths of tubing are disclosed in prior patents,for example, U.S. Pat. Nos. 5,525,303; 5,730,943; and 6,095,572, thedisclosures of which are herein all incorporated by reference as iffully set forth herein. Often, a first internally threaded fitting sealsto a first component with a ferrule or similar sealing device. The firstfitting is threadedly connected through multiple turns by hand or by useof a wrench or wrenches to a second fitting having a correspondingexternal fitting, which is in turn sealed to a second component by aferrule or other seal. Disconnecting these fittings for componentreplacement, maintenance, or reconfiguration often requires the use of awrench or wrenches to unthread the fittings. Although a wrench orwrenches may be used, other tools such as pliers or other gripping andholding tools are sometimes used. In addition, the use of suchapproaches to connect components of an LC system often results indeformation or swaging of a ferrule used to provide a leak proof seal oftubing to a fitting or component. This often means that the ferrule andtubing connection, once made, cannot be reused without a risk ofintroducing dead volumes into the system. In addition, such approachesmay involve crushing or deformation of the inner diameter of the tubing,which may adversely affect the flow characteristics and the pressures ofthe fluid within the tubing.

Another approach to provide a connection in an LC system involvesproviding a fitting assembly that uses a combination of components,including two separate ferrules. Such an approach is consideredundesirable because by requiring two places for the ferrules to provideleak proof seals, it provides two places where the fluid to be analyzedmay leak, as well as where dead volumes may be provided. In addition,this approach involves the use of additional components, which can costmore and also increase the time and effect to assemble them to make aconnection or disassemble them when disconnecting tubing from acomponent or other fitting assembly.

There exists a need for fluidic fittings that are more reliable and haveincreased performance, which can be accomplished by applying a specificamount of torque to a fluidic fitting. The long used standard of “fingertight” when applying torque introduces a great deal of variation intothe process. This results in fittings being under tightened, whichcauses leaks, or potentially over-tightened (with a tool), which canresult in damage to fittings and ports. In general a torque limitingfitting may be preferred over the use a torque tool (such as a torquewrench) since torque tools require specific designs to allow access tospecific fittings, employee training, additional assembly time, andassociated costs (e.g., tool purchase and periodic calibration).

U.S. Pat. No. 5,183,140 discloses a general torque limiting mechanism,which comprises two rotatable members, one of which is the drivingmember and the other of which is the driven member. One of the membersincludes a single radial projection extending from a central hub thatengages a recessed area on the other member. Below the torque limit theprojection engages the recessed area and allows the driving member todrive the driven member, but above the torque limit the projectiondisengages the recessed area and prohibits the driving member fromdriving the driven member. U.S. Pat. No. 7,984,933 discloses a torquelimiting fitting, which also comprises two rotatable members, one ofwhich is the driving member and the other of which is the driven member.One of the members includes a lever extending from a central hub thatengages an abutment on the other member. Below the torque limit thelever engages the abutment and allows the driving member to drive thedriven member, but above the torque limit the lever deflects from theabutment and prohibits the driving member from driving the drivenmember. However, the radial projection and the lever are only supportedon one end, which can result in inconsistency in the torque limit andgenerally lower maximum torque values.

It will be understood by those skilled in the art that, as used herein,the term “LC system” is intended in its broad sense to include allapparatus and components in a system used in connection with a liquidchromatography system, and that the discussion of fittings in thecontext of LC systems is exemplary, as the invention may apply beyond LCsystems to gas and ion chromatography, as well as or in vitro diagnosticor environmental analysis, and in other analytical instruments andsystems, and may be made of only a few simple components or made ofnumerous, sophisticated components which are computer controlled or thelike. Those skilled in the art will also appreciate that an LC system isone type of an analytical instrument (AI) system. Those skilled in theart will appreciate that much of the foregoing discussion with respectto LC systems also has application to other types of AI systems andmethods.

Conventional fitting assemblies in certain applications will usefittings that are screwed into ports. Such conventional fittings do notinclude an energizing member to supply a constant force, which may beneeded if the fitting assembly or one or more of its components changes(e.g., such as via creep) over time.

Experience teaches that most users of equipment involving such fluidicconnections do not like using tools to install fittings. Most prefer tomake fluidic connections by hand and to disconnect the fluidicconnections, once made, by hand. Moreover, most users do not like to berequired to apply torque to threaded fittings (whether by hand or by useof a tool) to a specific torque value or range or values, often due tothe time or extra equipment needed to do so. Also, in many situations,it is not entirely clear if an operator has applied enough or too muchtorque in making up a connection until the AI system is in operation anda leak occurs or the pressure of the fluid flowing through a tube causesits extrusion from the port.

In many conventional fluidic connections, it can be difficult todetermine if the fitting assembly is correctly or fully installed ornot, unless and until a leak or burst occurs. For example, a fittingassembly may appear fine because it is partially screwed into a threadedport, but in fact is not fully engaged to provide a seal at a desiredpressure range for a given application because the user failed to applysufficient torque when making the connection. Conventional fluidicconnections and fitting assemblies do not provide an easily observableindication of whether the fitting assembly is fully connected.

Attempts have been made to try to address some of the drawbacks andissues with conventional fitting assemblies and connections. Forexample, attempts have been made to develop approaches intended toprovide fittings which may be quickly connected to and disconnected froma component. In U.S. Pat. No. 5,803,512, titled “Tube Quick Connect toFemale Socket,” issued to Hollnagel on Sep. 8, 1998, a quick connectassembly is described, which relies on a pair of slanted arms, theradial deflection of which allows a fitting to connect and disconnectwith a female socket member. Likewise, U.S. Pat. No. 4,834,423, titled“Quick Connect Fluid Fitting Assembly,” issued to DeLand on May 30,1989, describes a quick-connect fitting assembly which relies on aretainer means including at least one finger which can connect andrelease in interlocking engagement with a socket. Similarly, U.S. Pat.No. 8,448,994, titled “Latch Assembly For Joining Two Conduits,” issuedto Pisula et al. on May 28, 2013, describes a latch assembly for theconnection of conduits, where the connection is established byengagement of a feature within a slot and is disengaged by a releasebutton. U.S. Pat. No. 6,497,433, titled “Coupling Assemblies forProviding Fluid Connection,” issued to Ketcham on Dec. 4, 2002, alsodiscloses a retainer feature, in this case relying on at least twolocking beams to engage and release a conduit to and from a femaleconnector body.

Other attempts have been made to provide various connection assemblies.For example, U.S. Pat. No. 4,781,399, titled “Quick-Connect CouplingHaving Improved Seal” and issued on Nov. 1, 1988, describes a connectionassembly with a ball detent and seal for a household faucet or the like.U.S. Pat. No. 4,135,745, titled “Quick Connect Fluid Fitting” and issuedon Jan. 23, 1979, describes a connection assembly with an expandableclip on a tube intended to allow tubing to be attached to a housing witha preassembled nut. U.S. published patent application No. 2014/0102561,published on Apr. 17, 2014 and titled “Quick-Release Connectors andConnection Assemblies for Fluidic Coupling” describes the use of aplunger body for use with a check valve. U.S. Pat. No. 7,695,020, issuedon Apr. 13, 2010, and titled “Coupling with Latch Mechanism,” describesa coupling apparatus with a mechanical latch assembly with a latchplate. U.S. Pat. No. 6,361,687, titled “Quarter Turn Quick ConnectFitting” and issued on Mar. 26, 2002, describes a fitting assembly witha quarter turn nut which cooperates with a fitting with pins. U.S. Pat.No. 6,149,127, issued on Nov. 21, 2000, and titled “Spring LoadedCompression Valve Fitting” describes a valve fitting to be coupled to avalve body and cause a compression member to limit the pressure exertedon the valve body. U.S. Pat. No. 5,951,063, titled “Quick Connector withSnap-On Retainer Having Enhanced Engagement” and issued on Sep. 14,1999, describes a retainer with lock projections for releasably lockingtogether male and female components. It is believed that none of theseprior attempts have provided a connection assembly which can quickly andeasily be used by an operator to connect or disconnect tubing withoutthe need for any torque or any rotation of a component of the assembly,and without any tools, and yet provide a sealing engagement for highpressure applications in a cost effective manner. U.S. Pat. Nos.4,781,399, 4,135,745, 7,695,020, 6,361,687, 6,149,127, and 5,951,063,and U.S. published patent application No. 2014/0102561, are herebyincorporated by reference as if fully set forth herein.

None of these references disclose a means for supplying constant axialforce on the fitting as the fitting changes over time (e.g., the fittingmaterial creeps). Such a force can be advantageous to maintain theintegrity of the tube-to-port seal as the fitting assembly creeps. Ifthe fitting assembly creeps and there is no compensating axial force,this could result in leakage at the point of the tube-to-port interfaceand could thus compromise the fitting assembly. In addition, none of thereferences noted above disclose a tube-to-port seal wherein the seal isgenerated solely by the tube face, without the need for torque or extracomponents (e.g., ferrules). Moreover, conventional fitting assembliesfail to provide a connection system in which an operator can easilydetermine from visual examination that a seal has been obtained when aconnection has been made.

SUMMARY OF THE INVENTION

In one embodiment of the present disclosure, a fluidic connectionassembly is provided which includes a body having a first end and asecond end, and having a plurality of sides, and further having a bottomportion with a plurality of latch portions, each corresponding to one ofthe sides of the body, wherein the latch portions are adapted to beretained in a port when inserted therein, said body further having apassageway therethrough adapted to receive a tube extending through saidbody, said body further having a plurality of hinges, each of the hingescorresponding to one of the sides of said body, wherein the sides andthe corresponding latch portions of said body are adapted to moveinwardly towards the longitudinal axis of the body when a force isexerted thereon, thereby allowing the body to be disconnected from theport, and wherein the first end of said body comprises an extensionmember and the second end of said body is adapted to cooperate with aspring for exerting a force against a bottom of the tube. The fluidicconnection assembly can further comprise a second spring in an interiorportion of said body such that the second spring exerts a force againstthe plurality of sides of said body towards the exterior of said bodyand away from the longitudinal axis of the body. The extension and saidbody can be integral or can be formed from separate pieces. The secondspring can be one or more of any of a number of different types ofsprings, such as a band spring, a cantilever spring, an elastomericspring, or the like.

In some embodiments of the present disclosure, the fluidic connectionassembly can also include a spring and a tube extending through theextension member, through the body, and through the spring, wherein thespring is located between the second end of said body and one end ofsaid tube. In addition, the end of the tube can comprise a flange and/orthe spring can comprise a coiled spring. In some embodiments, theextension portion may include a plurality of toroidal members and/or aspine member, and the spine member can be provided by a wire having apolymeric coating. Some embodiments may further include a backup ringlocated between the spring and the flange of said tube, wherein thebackup ring may be used to help concentrate the force from said springon the flange in an area smaller than the total surface area of theflange. The backup ring can be radially tapered to concentrate axialforce on a smaller area of the tube second end, and the end of the tubemay comprise a reduced surface area. The fluidic connection assembly inaccordance with the present disclosure may have a body which comprisesany one or more of various materials, which may include metal, such assteel, including stainless steel, aluminum, titanium, as well aspolymeric materials such as polyetheretherketone (PEEK),polyoxymethylene (POM) such as available under the mark DELRIN and knownsometimes as acetal, RADEL brand polyphenylsulfone (PPSU), ULTEM brandpolyetherimide (PEI), polyetherketoneketone (PEKK), polyaryletherketone(PAEK), polyethylene, polypropylene, polyvinylchloride, acrylic, and/orother materials, such a fused silica, silica borite, PEEKsil, and thelike.

In some embodiments of the present disclosure, methods are provided,including a method of making a connection which includes the steps ofproviding a tube having first and second ends, wherein the second endcomprises a flange, inserting the first end of the tube through a backupring, a spring, and a body, wherein said body comprises a first end anda second end, a plurality of sides, and a bottom portion having aplurality of latch portions, each corresponding to one of the sides,wherein the latch portions are adapted to be retained in a port wheninserted therein, said body further having a passageway therethroughadapted to receive a tube extending through said body, said body furtherhaving a plurality of hinges, each of the hinges corresponding to one ofthe sides of said body, wherein the sides and the corresponding latchportions of said body are adapted to move inwardly towards the center ofthe body when a force is exerted thereon, and wherein the second end ofsaid body is adapted to cooperate with a spring for exerting a forceagainst a bottom of the tube, and inserting a least a portion of thebody, including at least the latch portions of the body, into a port,wherein the latch portions are retained within the port. In at leastsome embodiments, such methods do not require the application of anytorque, or the use of a threaded engagement of two members, or therelative rotation of two threaded members to provide a threadedengagement.

In some embodiments, methods of disconnecting a tube connected to a portare provided. Such methods may include the steps of exerting a force ona plurality of sides of a body in a fluidic connection assembly, whereinthe fluidic connection comprises a tube having first and second ends,wherein the second end comprises a flange, and wherein at least aportion of the tube between the second end and the first end extendsthrough the body, wherein the body has a first end and a second end, anda bottom portion with a plurality of latch portions, each correspondingto one of the sides, said body further having a passageway therethroughadapted to receive the tube, said body further having a plurality ofhinges, each of the hinges corresponding to one of the sides of saidbody, wherein the sides and the corresponding latch portions of saidbody are adapted to move inwardly towards the center of the body when aforce is exerted thereon, thereby moving the latch portions of the bodyinwardly, and then pulling the assembly from the port. In someembodiments of such methods, the steps do not require the application ofany torque to a component of the assembly.

In some embodiments of the present disclosure, an analytical instrumentsystem is provided which includes a fluidic connection which in turnincludes a body having a first end and a second end, and having aplurality of sides, and further having a bottom portion with a pluralityof latch portions, each corresponding to one of the sides, wherein thelatch portions are adapted to be retained in a port when insertedtherein, said body further having a passageway therethrough adapted toreceive a tube extending through said body, said body further having aplurality of hinges, each of the hinges corresponding to one of thesides of said body, wherein the sides and the corresponding latchportions of said body are adapted to move inwardly towards the center ofthe body when a force is exerted thereon, thereby allowing the body tobe disconnected from the port, and wherein the first end of said bodycomprises an extension member and the second end of said body is adaptedto cooperate with a spring for exerting a force against a bottom of thetube. The AI system may further include a spring and a tube extendingthrough the extension member, through the body, and through the spring,wherein the spring is between the second end of said body and one end ofsaid tube. In addition, the spring may exert a force against the flangeof the one end of said tube and maintain a sealing engagement. In someembodiments, the AI system may be used for in vitro applications. In atleast some of the embodiments of the present disclosure, the fittingassembly, once connected, may provide a seal at a fluid pressure of atleast 200 psi, 500 psi, 1000 psi, 2500 psi, 5000 psi, 10000 psi, and20000 psi. In addition, in some embodiments, the fitting assembly canprovide a visual cue to an operator to indicate that a proper connectionhas been made for an intended application. Moreover, the presentdisclosure provides apparatus and methods which allow an operator tomake up a fluidic connection, or to disconnect a fluidic connection, buthand and without the need for pliers, wrenches, or other tools, andwithout the need for application of a torque, such as is the case when aconnection is made which requires the relative rotation of two threadedmembers. The connection assembly can be used in connection with any oneof a number of ports and/or components in any one or more AI system,including in connection with components including any one or more of thefollowing: a pump, a column, a filter, a guard column, a valve, adetector, a pressure regulator, a reservoir, a degasser, a debubbler, aunion, a tee, a cross, an adapter, a splitter, a sample loop, or aconnector.

In certain embodiments in accordance with the present disclosure, anassembly body for connecting a tube to a port comprising a body isprovided, which has a passageway therethrough and has located thereinmeans for exerting an axial force to bias an end of a tube towards aface of a port, and further has means for removably and securelyattaching said body to the port, wherein the means for removably andsecurely attaching said body are adapted to allow a user to selectbetween a first position and second position of the means for removablyand securely attaching said body to thereby attach said body to the portin the first position and to disengage said body from the port in thesecond position. The end of the tube may further comprise a tip, and theport may be formed by a recess formed in an adapter attached to anothercomponent, such as an adapter connected to the end of a column, or maybe a port which is part of a manifold, a pump, a valve, a column, afilter, a guard column, a detector, a pressure regulator, a reservoir, adegasser, a debubbler, a union, a tee, a cross, a splitter, a sampleloop, a connector, or another component in an AI instrument or system.The means for exerting an axial force may comprises a spring, includingwithout limitation a coiled spring. The means for removably and securelyattaching may comprise radial projections on said body adapted to engagewith portions of the port, such as radial projections shoulders whichlatch together when connected, or may comprise a ball detent mechanism.The body is adapted to removably and securely attach to a port withaxial loading, such as by an operator by hand or by automated means,without any torque being applied to said body or the port, and withoutrequiring that the body or the port be turned. The tube, the body, andthe port assembly may comprise biocompatible materials. In addition, theport may comprise a first inner diameter at an opening thereof and abottom surface with a reduced surface area for contact with the end ofthe tube, wherein the bottom surface has an outer diameter less than thefirst inner diameter of the port. The portion of the tube located withinthe body may have a first diameter and the end of the tube may comprisea bottom surface having an outer diameter less than the first outerdiameter of the tube, thereby concentrating the force exerted at thesealing location and allowing a seal to be formed capable of a leak-freeseal and preventing extrusion of the tube from the port withoutrequiring such force to be applied to connect the assembly together byan operator. In addition, the tube may have a tip which has a first endwith a first outer diameter and the tip also has a portion thereof witha second outer diameter which is greater than the first outer diameter.The port may be comprised of a combination of two or more distinctcomponents, such as if the port comprises a bottom surface of a firstcomponent and an recess extending from the bottom surface is provided bya second component.

In certain embodiments, a fluidic connection assembly for high pressureapplications is provided which comprises a connector assembly having aconnector and having means for allowing an operator to select between afirst position of said connecter and a second position of saidconnector, wherein in the first position said connector is adapted to beinserted into or removed from a port, and in the second position saidconnector is adapted to be retained in the port, and wherein saidconnector has a passageway therethrough adapted to receive a portion ofa tube, and means located within said connecter for exerting a force ona portion of tube located within the passageway of said connector whensaid connector is in the second position, wherein said connector isadapted to be connected to the port without any torque and withoutturning either said connector or the port, and wherein said connecterassembly and said means for exerting a force on a portion of a tube areadapted to sealingly engage the tube in the port when a fluid flowsthrough the tube. The means for allowing an operator to select between afirst position and a second position may comprise a plurality of tabseach connected to a corresponding radial projection which moves radiallyinward or outward when the tabs are moved radially inward or outward,and such movement may correspond to each other (i.e., inward movement ofthe projections when the tabs move inward), or may be different from oneanother (i.e., inward movement of the projections when the tabs aremoved outwardly). The means for allowing an operator to select between afirst position and a second position may comprise a collar and balldetent. The means for exerting a force may comprises a spring, and themeans may be preselected so that the force adapted to be exerted is aforce of at least a preselected amount, or within a preselected range.The means for exerting a force and said connector assembly may beadapted to sealingly engage one end of the tube when a fluid flowsthrough the tube at pressures up to 5,000 psi, 10,000 psi, 15,000 psi,20,000 psi, and/or 25,000 psi.

In certain embodiments of the present disclosure, a fluidic connectionassembly is provided and which comprises a body having a first end and asecond end, and having a plurality of sides, and further having a bottomportion with a plurality of latch portions, each corresponding to one ofthe sides, wherein the latch portions are adapted to be retained in aport when inserted therein, said body further having a passagewaytherethrough adapted to receive a tube extending through said body, saidbody further having a plurality of sides each having a slot therein,each slot corresponding to one of the sides of said body, wherein eachside of said body which has a slot does not have a corresponding latchportion, wherein the sides with corresponding latch portions of saidbody are adapted to move inwardly towards the center of the body when aforce is exerted thereon, thereby allowing said body to be disconnectedfrom the port, and wherein the second end of said body is adapted tocooperate with a spring for exerting a force against a bottom of thetube.

In addition, certain embodiments provide a method of connecting a tubeto a port of a component of an analytical instrument system, whichmethod comprises providing an assembly having a first end and a secondend, wherein the assembly is adapted to connect to a port of a componentof an analytical instrument system, providing a tube having a first endand a second end and extending through the assembly, and inserting,without any threaded engagement of the assembly with the port, at leasta portion of the second end of the tube and the second end of theassembly into the port so that a portion of the assembly is removablyheld in the port and provides a sealed connection between the second endof the tube and the port, wherein the sealed connection is leakproof atpressures of up to at least 2,500 psi.

In other embodiments, a fluidic connection assembly is provided whichcomprises a body having a first end and a second end, and having aplurality of tabs located between the first and second ends, whereineach of the plurality of tabs extends radially outwardly from said body,and wherein the second end of said body further has a plurality ofradially inwardly extending latch portions, each corresponding to one ofthe tabs, wherein the latch portions are adapted to engage and retain afirst end of a port body when the first end of the port body is betweenthe latch portions of said body and the latch portions are in a closedposition, and the latch portions are adapted to move radially outwardlywhen the corresponding tabs move radially inwardly, said body furtherhaving a passageway therethrough adapted to receive a tube extendingthrough said body, and a spring located in said body and having a firstend proximal the first end of said body and a second end adapted toexert a force to urge one end of the tube against at least a portion ofa port in the port body. The fluidic connection assembly may furthercomprise a spring and a tube extending through the extension member,through the body, and through the spring. The end of said tube mayfurther comprise a PEEK tip, and the spring may comprise a coiledspring. In addition, the assembly may further comprise a boss in theport of the port body which has a diameter smaller than the outerdiameter of the tube. The port may comprise a port within an AI systemcomponent, including at least one of a union, tee, cross, pump, valve,column, guard column, manifold, or detector.

In some embodiments, a fluidic connection assembly is provided whichcomprises a first body having a passageway therethrough and having firstend and a second end, and having a plurality of tabs defining the secondend, wherein a middle portion of said first body has a plurality ofradially inwardly extending shoulder portions, each corresponding to oneof the tabs, wherein each of the shoulder portions are adapted to moveradially inwardly when a corresponding tab is moved radially inwardly, asecond body having a first end and a second end and located within saidfirst body, wherein the first end of said second body abuts the interiorsurface of the first end of said first body, said second body furtherhaving a plurality of middle portions, each adapted to move radiallyinwardly when a corresponding shoulder portion of said first body movesradially inwardly, and further having latch portions proximal the secondend of said second body, wherein the latch portions are adapted to moveradially inwardly when the middle portions move radially inwardly, andwherein the latch portions are adapted to removably engage with radiallyinward projections at a first end of a port body in an AI system, saidsecond body further having a passageway therethrough adapted to receivea tube extending through said second body, and a spring located in saidsecond body and having a first end proximal the first end of said secondbody and a second end adapted to exert a force to urge one end of thetube against at least a portion of a port in the port body. The assemblymay further comprise a spring and a tube extending through said spring,and the end of said tube may comprise a PEEK tip. In addition, theassembly may further comprise a boss in the port of the port body whichhas a diameter smaller than the outer diameter of the tube. The port maycomprise a port within an AI system component, including at least one ofa union, tee, cross, pump, valve, column, guard column, manifold, ordetector.

In certain embodiments, a fluidic connection assembly is provided whichmay comprise a body having a first end and a second end, each of thefirst and second ends having openings therein, and having a hollowportion therein, a first spring located at least partially within thehollow portion of said body, and having a first end abutting an interiorsurface of the hollow portion of said body, a latch member having anopening therethrough and having a base portion and a top portion,wherein the base portion of said latch member is adapted to fit at leastpartially within said body, and wherein the top portion has one or moreprojections adapted to be held in recesses on an exterior portion ofsaid body, and a cap member having an opening therethrough and havingfirst and second sides and an extension on the first side of said capmember which is adapted to extend into at least a portion of the hollowportion of said body and to hold said latch member and said cap membertogether, wherein the second side of said cap member is adapted to beattached to the second end of said body, and wherein said latch memberis adapted to engage with and securely hold an adapter when a portion ofone end of the adapter is inserted into the opening of said cap memberand the opening of said latch member, and wherein each of said body,said spring, said latch member, and said cap member are adapted toreceive at least a portion of tubing therethrough. In addition, thefirst spring may be in a compressed state when said cap member, saidlatch member, and said body are assembled together and said first springis located entirely within the hollow portion of said body. The fluidicconnection assembly may further comprise a second spring, wherein saidsecond spring is located within the hollow portion of said body andadjacent to at least one side of said latch member. The second springmay be adapted to push said latch member so that a radially inwardprojection of said latch member is pressed against a portion of saidadapter. The adapter and the inward projection of the latch member mayhave cooperating ramps or angled portions to allow for easier engagementof the adapter and the inward projection. The fluidic connectionassembly may further comprise a tube having one end which extendsthrough the first and second ends of said body, said first spring, saidlatch member, and extends out of the opening of the second side of saidcap member. The first spring may exert a force which urges the end ofsaid tube away from said body. The fluidic connection assembly mayfurther comprise a washer or disc located within said body and adjacentto a second end of said first spring, a sleeve member surrounding atleast a portion of said tube, wherein said sleeve member has a first endand a second end, and wherein said first spring exerts a force on saidwasher and said washer exerts a force on said sleeve, and said sleeveexerts a force on said tube which urges the end of said tube away fromsaid body. The assembly may further comprise a tip surrounding a portionof the tube and at least a portion of which is surrounded by the sleeve,wherein the force exerted on the sleeve is transferred to one end ofsaid tip to urge said tip against a surface of a port. The port maycomprise a port within an AI system component, including at least one ofa union, tee, cross, pump, valve, column, guard column, manifold, ordetector.

In certain embodiments, a fluidic connection assembly is provided whichmay comprise a body having a first end and a second end, each of thefirst and second ends having openings therein, and having a hollowportion therein, a first spring located at least partially within thehollow portion of said body, and having a first end abutting an interiorsurface of the hollow portion of said body, a latch member having anopening therethrough and having a base portion, and a cap member havingan opening therethrough and having first and second sides, and which isadapted on one side to surround a portion of one end of the body, withthe cap further having a base portion which is adapted to receive andhold a second spring therein, with the cap further adapted to receiveand hold at least a portion of the latch member therein, with the latchmember further adapted to engage with and securely hold an adapter whena portion of one end of the adapter is inserted into the opening of saidlatch member, and wherein each of said body, said spring, said latchmember, and said cap member are adapted to receive at least a portion oftubing therethrough. In addition, the first spring may be in acompressed state when said cap member, said latch member, and said bodyare assembled together and said first spring is located entirely withinthe hollow portion of said body. The fluidic connection assembly mayfurther comprise a second spring, wherein said second spring is locatedwithin the base portion of said cap and adjacent to at least one side ofsaid latch member. The second spring may be adapted to push said latchmember so that a radially inward projection of said latch member ispressed against a portion of said adapter and/or into an annular notchof the adapter. The adapter and the inward projection of the latchmember may have cooperating ramps or angled portions to allow for easierengagement of the adapter and the inward projection. The fluidicconnection assembly may further comprise a tube having one end whichextends through the first and second ends of said body, said firstspring, said latch member, and extends out of the opening of the secondside of said cap member. The first spring may exert a force which urgesthe end of said tube away from said body. The fluidic connectionassembly may further comprise a washer or disc located within said bodyand adjacent to a second end of said first spring, a sleeve membersurrounding at least a portion of said tube, wherein said sleeve memberhas a first end and a second end, and wherein said first spring exerts aforce on said washer and said washer exerts a force on said sleeve, andsaid sleeve exerts a force on said tube which urges the end of said tubeaway from said body. The assembly may further comprise a tip surroundinga portion of the tube and at least a portion of which is surrounded bythe sleeve, wherein the force exerted on the sleeve is transferred toone end of said tip to urge said tip against a surface of a port. Theport may comprise a port within an AI system component, including atleast one of a union, tee, cross, pump, valve, column, guard column,manifold, or detector.

In certain embodiments a method of connecting a tube to a port isprovided, with the method comprising the steps of providing a tubehaving first and second ends, inserting the first end of the tubethrough a fluidic connection assembly which may comprise a body having afirst end and a second end, each of the first and second ends havingopenings therein, and having a hollow portion therein, a first springlocated at least partially within the hollow portion of said body, andhaving a first end abutting an interior surface of the hollow portion ofsaid body, a latch member having an opening therethrough and having abase portion and a top portion, wherein the base portion of said latchmember is adapted to fit at least partially within said body, andwherein the top portion has one or more projections adapted to be heldin recesses on an exterior portion of said body, and a cap member havingan opening therethrough and having first and second sides and anextension on the first side of said cap member which is adapted toextend into at least a portion of the hollow portion of said body and tohold said latch member and said cap member together, wherein the secondside of said cap member is adapted to be attached to the second end ofsaid body, and wherein said latch member is adapted to engage with andsecurely hold an adapter when a portion of one end of the adapter isinserted into the opening of said cap member and the opening of saidlatch member, and wherein each of said body, said spring, said latchmember, and said cap member are adapted to receive at least a portion oftubing therethrough, inserting a least a portion of tube into an adapterand inserting at least a portion of one end of the adapter into thesecond end of said body and having the projection of said latch memberengage with a portion of the adapter to securely hold the adapter andthe latch member together, and engaging the other end of the adapterwith a port, such as by threadably engaging threads on the adapter withthreads of the port.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is an isometric view of a fluidic connector assembly inaccordance with an embodiment of the present disclosure.

FIG. 2 is a front view of a fluidic connector assembly in accordancewith an embodiment of the present disclosure.

FIG. 3 is a cross-sectional view of the fluidic connector assembly shownin FIG. 2 taken along line C-C.

FIG. 4A is an enlarged cross-sectional view of a portion of the fluidicconnector assembly shown in FIGS. 2 and 3.

FIG. 4B is an isometric view of a band spring useful in an embodiment ofthe present disclosure.

FIG. 5 is a side view of a fluidic connector assembly in accordance withan embodiment of the present disclosure.

FIG. 6 is a cross-sectional view of the fluidic connector assembly shownin FIG. 5 taken along line D-.

FIG. 7 is a cross-sectional view of an embodiment of a fluidic connectorassembly which is connected to a port.

FIG. 8 is an enlarged cross-sectional view of a portion of the fluidicconnector assembly shown in FIG. 8.

FIG. 9 is a cut-away view of an embodiment of a fluidic connectorassembly in accordance with the present disclosure.

FIG. 10 is a cross-sectional view of the fluidic connector assemblyshown in FIG. 9.

FIG. 11 is a cross-sectional view of a fluidic connector assembly inaccordance with an embodiment of the present disclosure, and is shown ina connected state.

FIG. 12 is an enlarged, cross-sectional view of a portion of the fluidicconnector assembly shown in FIG. 11

FIG. 13 is an enlarged, cross-sectional view of a portion of a fluidicconnector assembly in accordance with an embodiment of the presentdisclosure.

FIG. 14 is an enlarged, cross-sectional view of a portion of a fluidicconnector assembly in accordance with an embodiment of the presentdisclosure.

FIG. 15 is an enlarged, cross-sectional view of a portion of a fluidicconnector assembly in accordance with an embodiment of the presentdisclosure.

FIG. 16 is an enlarged, cross-sectional view of a portion of a fluidicconnector assembly in accordance with an embodiment of the presentdisclosure.

FIG. 17 is a cross-sectional view of a fluidic connector assembly inaccordance with one embodiment of the present disclosure, and is shownin a connected state.

FIG. 18 is an enlarged cross-sectional view of a portion of a fluidicconnector assembly in accordance with one embodiment of the presentdisclosure, and is shown in a connected state.

FIG. 19 is another enlarged cross-sectional view of a portion of thefluidic connector assembly shown in FIG. 18 in accordance with oneembodiment of the present disclosure, and is shown in a connected state.

FIG. 20 is another enlarged cross-sectional view of a portion of thefluidic connector assembly shown in FIG. 17 in accordance with oneembodiment of the present disclosure, and is shown in a connected state.

FIG. 21 is an isometric view of another alternative embodiment of anassembly in accordance with the present disclosure.

FIG. 22 is a side view of the assembly of FIG. 21.

FIG. 23 is a cross-sectional view of the assembly of FIG. 21 taken alongline E-E of FIG. 22.

FIG. 24 is another side view of the assembly of FIG. 21.

FIG. 25 is a cross-sectional view of the assembly of FIG. 21 takingalong line F-F of FIG. 24.

FIG. 26 is a cross-sectional view of a connection assembly in accordancewith an embodiment in accordance with the present disclosure.

FIG. 27 is a cross-sectional view of a connection assembly and a portconfiguration in accordance with an embodiment in accordance with thepresent disclosure.

FIG. 28 is a cross-sectional view of a connection assembly and a portconfiguration in accordance with an embodiment in accordance with thepresent disclosure.

FIG. 29 is a cross-sectional view of a connection assembly and a portconfiguration in accordance with an embodiment in accordance with thepresent disclosure.

FIG. 30 is a cross-sectional view of a connection assembly in accordancewith an embodiment in accordance with the present disclosure.

FIG. 31 is a cross-sectional view of a connection assembly and a portconfiguration in accordance with an embodiment in accordance with thepresent disclosure.

FIG. 32 is an exploded view of a connection assembly of an alternativeembodiment in accordance with the present disclosure.

FIG. 33 is a partial cross-sectional view of the connection assembly ofFIG. 32 in a connected configuration in accordance with the presentdisclosure.

FIG. 34 is an exploded view of a connection assembly of an alternativeembodiment in accordance with the present disclosure.

FIG. 35 is a partial cross-sectional view of the connection assembly ofFIG. 33 in a connected configuration in accordance with the presentdisclosure.

FIG. 36 is a partial cross-sectional view of a tubing assembly inaccordance with one embodiment of the present disclosure.

FIG. 37 is an enlarged partial cross-sectional view of the tubingassembly of FIG. 36.

FIG. 38A is a frontal view of a tip of the tubing assembly of FIGS. 36and 37.

FIG. 38B is a side view of a tip of the tubing assembly of FIGS. 36 and37.

FIG. 38C is a cross-sectional view of the tip of the tubing assembly ofFIGS. 36 and 37.

FIG. 39 is a cross-sectional view of a quick connect/disconnect assemblyin a connection assembly in one embodiment of the present disclosure.

FIG. 40 is an exploded view of the assembly of FIG. 39.

FIG. 41 is a view of the assembly of FIGS. 39 and 40 shown in anexploded cross-sectional view with an adapter and a union in anembodiment of the present disclosure.

FIG. 42 is an exploded view of a fluidic connection assembly of oneparticular embodiment in accordance with the present disclosure.

FIG. 43 is a partially exploded three-dimensional view of a fluidicconnection assembly of one particular embodiment in accordance with thepresent disclosure.

FIG. 44 is a three-dimensional view of the fluidic connection assemblyof FIG. 43 in an assembled state.

FIG. 45 is a cross-sectional view of the fluidic connection assembly ofFIG. 43.

FIG. 46 is a cross-sectional view of the fluidic connection assemblyshown in FIG. 45 as shown in an assembled state.

DETAILED DESCRIPTION

FIG. 1 provides an isometric view of a fluidic connector assembly 1 inaccordance with one embodiment of the present disclosure. As shown inFIG. 1, the fluidic connector 1 and its components are assembled. Asshown in FIG. 1, the assembled fluidic connector assembly 1 includestubing 5, a top extension 10, a body 15, a spring 20, and a bottomflange 25. In this particular embodiment, the tubing 5 extends throughpassageways adapted to receive the tubing 5 in each of the extension 10and body 15, and the tubing 5 extends through the center open area ofthe spring 20, which in this particular embodiment is a coiled spring.In this particular embodiment, the bottom flange 25 of the fluidicconnector assembly 1 is provided by one end of tubing 5. Each of thesemain components of the fluidic connector 1 and their use and operationare described in more detail below.

Still referring to FIG. 1, the extension portion 10 includes a member 30which extends longitudinally on one side of the extension 10 from a topend to a base member 36. Extension member 10 also includes a series oftoroidal, or ring-like, members 32. As shown in FIG. 1, the toroidalmembers 32 generally form rings around, and are generally disposedperpendicular to, the longitudinal axis of the extension 10. At the topend of the extension member 10 is a top toroidal member 34, which islarger in size than the other toroidal members 32 of the extension 10.Those skilled in the art will appreciate that the toroidal members 32may vary in size, shape, and number as may be desired. In addition, thetoroidal members 32 could be replaced by a solid column (not shown) ifdesired, or by a column with slots, grooves, holes, or the like thereinas may be desired. The extension member 10 provides an advantage becauseit provides relief to tubing 5 to avoid unnecessary strains or stressesimposed on tubing 5 if twisted, grabbed, or otherwise manipulated by anoperator. The top extension 10 thus protects the tubing 5 to avoid wearand tear. To a lesser extent, an operator may grasp and manipulate theextension 10. Those skilled in the art will appreciate that theextension 10 can be provided in other forms as may be desired to providerelief for tubing 5, and will also appreciate that the assembly 1 can beprovided either with or without an extension 10, whether in the specificembodiment shown and disclosed herein or another form or shape. As alsoshown in FIG. 1, the base 36 of the extension 10 is located on top ofthe body 15. The body 15 includes a top portion 44, a face 41, a hinge33, and an indentation 40 on face 41, as well as a bottom member 42.Details regarding the body 15 and its features and operation areprovided below.

As shown in FIG. 1, located below the bottom member 42 of body 15 is aring portion 46 of the body 15 and, located below the ring portion 46,is a spring member 20. The spring member 20 in this particularembodiment can be a coiled spring of a desired material, length, andstrength. Those skilled in the art will appreciate that the material andsize of spring member 20 can be selected so that the spring member 20provides the desired amount of force, or biasing, when engaged (asdescribed in more detail below) in a fluidic connection in a desiredapplication. For example, one of skill would appreciate that it would beadvantageous to have the spring member 20 provide a greater biasingforce when the fluidic connector 1 will be used in an application with agreater fluidic pressure, than might be the case if the fluidicconnector 1 is to be used in an application with a relatively smallfluidic pressure. For example, not all fluidic connections in a given AIsystem used for a given application need operate under the same fluidpressure. The fluid pressures may change even in such situations fordifferent connections in the AI system. Moreover, a given AI system maybe used in an application in which lower fluid pressures are used thanmay be encountered in the same AI system when used for a differentapplication. For example, an LC system may be used for in vitroapplications with fluid pressures at 200 psi or less, yet this samesystem may also be used in other applications with higher pressures.

Also shown in FIG. 1 is a flange 25 of tubing 5. As can be seen, theflange 25 is located at the bottom of the fluidic connector 1, and belowthe bottom end of the spring 20. In the particular embodiment shown inFIG. 1, the flange 25 is one end of the tubing 5, and tubing 5 extendsfrom flange 25 through the spring 20, body 15, and extension 10. Theflange 25 provides an advantage because it is located between a port orother connector (not shown in FIG. 1) when the fluidic connector 1 isconnected to a port or other connector and the spring 20. This isuseful, for example, in systems involving biological samples, becausethe flange 25 and tubing 5 can be made of a biocompatible material andspring 20 can be made of metal if desired, as the spring 20 is notlocated in the flow path and thus cannot contaminate or otherwise affectthe fluid passing through the system in which the fluidic connector 1 isto be used.

Referring now to FIGS. 2 and 3, additional details regarding the fluidicconnector 1 and its components and features are shown. For ease ofreference, the same numbers are used in the various Figures for the sameitems. FIG. 2 is a “front” view of the fluidic connector 1, while FIG. 3is a cross-sectional view of the fluidic connector 1 taken along lineC-C of FIG. 2. Those skilled in the art will appreciate that terms suchas “top,” “bottom,” “front,” “back,” and the like as used herein are forconvenience in reference to the Figures, but in fact the fluidicconnector 1 can be used in any orientation, so such terms should not beconsidered limiting in any fashion but are instead merely for theconvenience of the reader.

As shown in FIGS. 2 and 3, the tubing 5 extends along a longitudinalaxis of the fluidic connector 1 as assembled. Each of the extension 10,body 15, and spring 20 are configured so that each has a passagewaythrough which the tubing 5 may extend. As shown in FIG. 3, the body 15has a first side 41 and a second side 43, and in this particularembodiment, each has an indentation 40. Those skilled in the art willappreciate that, although both sides 41 and 43 of the body 15 aresubstantially the same as one another, this need not be the case, suchas if a particular application in which the fluidic connector 1 is to beused presents a configuration such that a different shape or size ofbody 15 is more appropriate. As also shown in FIG. 3, a portion of thebase 36 of the extension 10 extends into an interior portion of the body15 at the top end of the body 15. Those skilled in the art willappreciate that the extension base 36 and the body 15 can be adapted toallow the extension base 36 to fit securely and/or releaseably in thetop end of the body 15. Alternatively, a portion of the extension base36 can be secured to the interior of the body 15, such as by use of aglue or adhesive, or the outer diameter of the portion of the base 36 tofit inside the interior portion of the body 15 can be substantially thesame size or slightly larger if the material of the extension base 36can be deformed as the inner diameter of the body 15 in the area adaptedto securely receive that portion of the extension base 36. Those skilledin the art will appreciate that, because the extension 10 need not beused if so desired, the base 36 need not form a portion of extension 10and, indeed, the connection assembly 1 and the body 15 do not requirethe use of the base 36. For example, the top of the body 15 in place ofa base 36 could instead be formed of the same material as the rest ofbody 15, or could be left open if desired. Those of skill will alsoappreciate that the body 15 and base 36 can be formed to allow asnap-fit connection, such that the base 36 and extension 10 can beremovably attached to the body 15 by an operator, or detached therefrom,as may be desired.

A hinge 33 is located at or near the top of the face 41 and at or nearthe top of face 43. The hinges 33 allow at least portions of the faces41 and 43 to move inwardly and outwardly with respect to thelongitudinal axis of the fluidic connector 1. Referring to FIG. 2, thehinge 33 at or near the top of the body 15 can be seen. In thisparticular embodiment, the hinge 33 defines the top of the face 41. Inaddition, a slot 23 on each side of the face 41 extends from each sideof the hinge 33 downward and defines the face 41. As shown in FIG. 2,the slots 23 further extend downwardly from face 41 on either side of afront face of the bottom member 42 of the body 15. As can be seen fromFIGS. 2 and 3, when the face 41 is pushed inwardly by an operatortowards the longitudinal axis of the assembly 1, the portion of face 41below hinge 33 can move inward because it is not fixed with respect tothe rest of body 15. In addition, it can be seen that the two slots 23join together near the bottom of the bottom member 42 of the body 15.The slots 23 are located and define the area of face 41 that can movewhen pressed such that the projections or latches 37 also move inwardlywith the face 41. It will be appreciated, such as from FIG. 3, forexample, that when the latches 37 both move inwardly, the distancebetween their outer edges decreases and provides a shorter length, thusallowing easy and quick insertion of the bottom member 42 of body 15into, or removal from, a port of an AI system component. Those skilledin the art will appreciate that, although slots 23 as shown in FIG. 2,for example, extend continuously from either side of hinge 33 and defineface 41 and then meet and join at or near the bottom of the bottommember 42, slots 23 can be configured differently if desired. Forexample, face 41 can be defined by a series of two or more slots (notshown) that are not continuous but yet extend in essentially the samepattern as shown in FIG. 2 for slots 23. Alternatively, instead of slots23, a weakened area of the body 15 may be provided, such as the casedescribed above for hinge 33 with a thinner width, to define face 41.

As shown in FIG. 3 (and as described in more detail below), a secondspring member 45 is located within body 15 in this embodiment. In anormal resting position (e.g., without any force applied to faces 41 and43 by an operator), the spring member 45 exerts a force against theinside surfaces of faces 41 and 43 and pushes them away from thelongitudinal axis and to a closed position, such as is shown in FIG. 2.When a force is exerted on the faces 41 and 43, pushing them towards thelongitudinal axis of the fluidic connector 1, the projections 37proximal the bottom of the body 15 move towards the longitudinal axis,thereby making it easier for an operator to quickly insert theprojections 37 (and thereby a portion of the fluidic connector 1) into aport or fitting (not shown in FIGS. 2 and 3), or to remove theprojections 37 (and thereby the fluidic connector 1) from a port orfitting.

Referring now to FIGS. 4A and 4B, additional details regarding thefluidic connector assembly 1 shown in FIGS. 2 and 3, and particularlywith respect to body 15, are shown and are described below. FIG. 4Aprovides an enlarged, cross-sectional view of a portion of the fluidicconnector 1, primarily the body 15. As shown in FIG. 4A, the tubing 5extends through passageways extending through the extension base 36 andthe body 15. As in FIG. 3, the body 15 in FIG. 4A includes a leftindentation 40 on the left face 41 and a right indentation 40 on theright face 43 of body 15. As in FIG. 3, a portion of the extension base36 is located within an interior portion of the body 15, at the top endof body 15, and a portion of the extension base 36 extends outward andexterior to the top end of the body 15. Located below the portion of theextension base 36 which is located within an interior portion of body 15is a second spring member 45.

As shown in FIG. 4A, the tubing 5 also extends through the spring member45 and along the longitudinal axis thereof. FIG. 4B provides anisometric view of the second spring member 45. As shown in FIG. 4B, thesecond spring member 45 in this particular embodiment is a band springwith a slot running the vertical length thereof. The second springmember 45 serves to bias or push the two faces 41 and 43 outwardly andaway from the longitudinal axis of the assembly 1, but is selected andadapted (such as by the selection of its shape, material(s) and size) sothat it can be deformed (from the position shown in FIGS. 4A and 4B)inwardly when a force is applied, such as when a user presses the twoindentations 40 on the two faces 41 and 43 towards the longitudinal axisof the fluidic connector 1. Those skilled in the art will appreciatethat the assembly 1 can be usefully used without the need for a spring45. However, the use of a spring 45 is believed to be useful in order tobias the faces 41, 43 outwardly and to thereby avoid potential problemswith fatigue of the material of the body 15. For example, if no spring45 is used and the body 15 and faces 41, 43 have been pushed inwardlynumerous times, the faces 41, 43 may tend to stay inwardly disposed,which may not be desired. The use of spring 45 thus can be used to helpprovide biasing of faces 41, 43 outwardly as may be desired.

Those skilled in the art will appreciate that human strength may vary,sometimes substantially. A fluidic connector assembly which is easilyand quickly operable by men and women, strong or weak, would beadvantageous. Such an assembly also would be advantageous if the abilityto obtain a sealed connection does not vary from user to user, and doesnot require a narrow range of force or torque to obtain a sealedengagement, and does not require the use of any tools. The fluidicconnector assembly 1 provides these advantages. For example, springmember 45 can be selected to require no more than an amount in a rangeof from two to five (e.g., three) pounds of force to be applied toindentations 40 to move the projections 37 inwardly enough to detach thefluidic connector 1 from a port. Almost all reasonably healthy andnon-disabled adult humans are believed able to exert such a force bypinching sides 41 and 43 together between a thumb and finger (andwithout requiring any tools). Moreover, the fluidic connector assembly 1of the present disclosure is advantageous because, once connected to aport, the fluidic connector assembly 1 provides a secure sealingengagement. An operator can easily visually check to be sure that theprojections 37 are held in a port; such a confirmation is all that isneeded for the operator to confirm that a seal has been obtained withthe connection. Moreover, the sides 41 and 43 will provide a visual cueand confirmation of a sealing engagement because, once the sealingengagement has been made, the latches 37 will be biased outwardly fromthe longitudinal axis, and so will the faces 41 and 43, with thelocation of the faces 41 and 43 easily visible to an operator.

Referring now to FIGS. 5 and 6, additional views of the fluidicconnector assembly 1 in connection with the embodiment described aboveare provided. FIG. 5 provides a side view of the fluidic connector 1, aswell as a cutline D-D, and FIG. 6 provides a cross-sectional view of thefluidic connector 1 as taken along line D-D as indicated in FIG. 5. Itwill be appreciated that additional details regarding the shapes andfeatures of the fluidic connector 1 can be seen. For example, FIG. 6provides a cross-sectional view showing spine member 30 of the extension10 on one side of the extension 10 with the series of rings or toroidalmembers 32 on the other side.

Those skilled in the art will appreciate that, depending on the intendedapplication, including without limitation the expected fluidicpressures, the nature of the fluids to be used, the nature of thesamples to be analyzed, and the like, the composition of the materialsused, as well as the specific shapes and sizes, of the variouscomponents and features of the fluidic connector 1 as shown anddescribed above can be varied. For example, in applications in whichbiocompatibility is desired, the tubing 5 can be made ofpolyetheretherketone (PEEK), polyaryletherketone (PAEK),polyetherketoneketone (PEKK), fluorinated ethylene propylene (FEP),ethylene tetrafluoroethylene (ETFE), polytetrafluoroethylene (PTFE),perfluoroalkoxy (PFA, also called perfluoroalkoxyethylene),polychlorotrifluoroethylene (PCTFE), polymer-sheathed fused silica (suchas PEEKSi1), fused silica, or silica borite, and can further include afiller material, which can include fibers, such as carbon fibers, glassfibers, nanofibers, and/or metallic fiber, depending on the pressuresand fluids involved. It is anticipated that the fluidic connector 1 willbe of particular usefulness in in vitro applications, such as those inwhich the fluidic pressures are about 200 psi or less, and in whichbiological materials are involved. In such situations, it is oftendesirable for the fluid path to contain only biocompatible materials. Inthe embodiment shown and described, however, this is achieved becausethe tubing 5 is the only component of the fluidic connector 1 touchingthe fluid. In the embodiment shown in FIGS. 1-6, then, the spring 20 andthe second spring member 45 can be made of metal, such as stainlesssteel, titanium, steel, nickel, nitinol. Alternatively, the spring 20and/or the second spring member 45 can be made of PEEK, PEI, and/orPPSU.

The extension 10 and body 15 can be made of any one of a number ofdifferent materials, including any one or more of the following: metal,such as steel, including stainless steel, aluminum, titanium, as well aspolymeric materials such as polyetheretherketone (PEEK),polyoxymethylene (POM) such as available under the mark DELRIN and knownsometimes as acetal, RADEL brand polyphenylsulfone (PPSU), ULTEM brandpolyetherimide (PEI), polyetherketoneketone (PEKK), polyaryletherketone(PAEK), polyethylene, polypropylene, polyvinylchloride, acrylic, and/orother materials, such a fused silica, silica borite, PEEKsil, and thelike. In one embodiment, the extension is made of one or more polymericmaterials such as polyetheretherketone (PEEK), polyoxymethylene (POM)such as available under the mark DELRIN and known sometimes as acetal,RADEL brand polyphenylsulfone (PPSU), ULTEM brand polyetherimide (PEI),polyetherketoneketone (PEKK), polyaryletherketone (PAEK), polyethylene,polypropylene, polyvinylchloride, acrylic, and/or other polymericmaterials, with a wire made of a metal located within the spine member30 and over molded with PEEK, although those skilled in the art willappreciate that any one of a number of additional or different polymersmay be used for this purpose. This particular embodiment of the spinemember 30 of the extension 10 allows an operator to bend or twist orotherwise shape the extension 10 or a portion thereof, and for thatportion of the extension so manipulated by an operator to retain itsshape indefinitely.

As noted above, the extension 10 can be optional. If included, extension10 can be integral with the body 15, or the extension 10 can be aseparate piece that is attached to the body 15. The extension 10 can bepermanently attached to the body, such as by glue, epoxy, or otheradhesive means, or can be attached such as by melting either or both ofextension 10 and body 15 and attaching them and then allowing thepolymeric material(s) to harden, or by sintering or other methods. Inaddition, the extension 10 and body 15 can be adapted so that theextension 10 can be removably attached to the body 15, such as byproviding latching means or a compression fit. For example, theextension 10 can be adapted so that its base end has a slightly largerarea than the top of the body 15 and can be compressed by a user, theninserted into the top of the body 15, such that when the operatorreleases the pressure on the extension 10 base end, it expands and isremovably secured to the body 15.

An embodiment of the operation of the fluidic connector 1 is nowdescribed with reference to FIGS. 2 and 3, although those skilled in theart will appreciate the operation of the fluidic connector 1 inconnection with all of the FIGs. An operator can assemble the fluidicconnector 1 by placing the tubing 5 through the center passageways ofthe spring 20, the body 15, and the extension 10. (As noted above, theextension 10 and body 15 can be securely joined together or can beremovably joined.) It will be appreciated that the spring 20, whencompressed, will exert a force against the flanged end 25 of the tubing5 and also against the bottom ring 46 of the body 15. This is describedin more detail below in connection with the use of the fluidic connector1 in a port.

As shown in FIG. 3, indentations 40 are located on opposing faces 41 and43 of the body 15. Although shown as circular, they need not be. It willbe appreciated that the spring 45 (shown in more detail in FIGS. 4A and4B) can be used to provide a force biasing the faces 41 and 43 towardsan outer or exterior position (i.e., away from the longitudinal axis offluidic connection 1). The faces 41 and 43 can be depressed by anoperator (without the use of tools), thus forcing the spring 45 tocompress towards the longitudinal axis of the fluidic connector 1. Asthe operator presses the faces 41 and 43 towards the longitudinal axisof the fluidic connector 1, the spring 45 compresses on opposing sidesas the faces 41 and 43 push the spring 45 inwardly towards thelongitudinal axis of the fluidic connector 1. As the spring 45 iscompressed inwardly, the bottom member 42 of the body 15 moves inwardlyfrom its previously biased position. When this happens, the latches orprojections 37 on the bottom member 42 also move inwardly and thus havea smaller outer diameter or length, thereby allowing quick insertioninto, and easy removal of the fluidic connector 1 from, a port or otherfluidic connection, such as in a component in an AI system.

By pressing faces 41 and 43 inwardly and inserting the bottom portion ofassembly 1 into a port, then releasing the pressure on faces 41 and 43,an operator can obtain a connection that is sealed and leak-proof, andprovides sufficient force to keep the sealed connection (even when afluid under high pressures, such as from about 200 psi to about 5000 psior so is flowing through the tubing 5) in place. An operator can make adesired connection in a LC or other analytical instrument system usingthe assembly 1 without the need for any torque, such as on a nut,without the need for a threaded connection, and without the need for anyadditional tools, such as a wrench or the like. Moreover, an operatorcan disconnect the assembly 1 from a port by pressing faces 41 and 43inwardly and pulling on the body 15 or other portion of the assembly 1and easily removing the assembly 1 from the port, again without the needfor any application of a torque, without the need for disconnecting athreaded engagement (such as if a nut with threads is used), and withoutthe need for any additional tools.

Referring now to FIGS. 7 and 8, an alternative embodiment of a fluidicconnector 701 is shown. In FIG. 7, the fluidic connector 701 includestubing 705 which extends along and around a longitudinal axis of thefluidic connector 701. The fluidic connector 701 includes an extensionmember 710, a body 715, a first ring 760, a second ring 765, a spring720, and a backup ring 775. The extension member 710 can besubstantially the same as the extension member 10 described above, andas shown in FIG. 7, the extension member 710 includes a series oftoroidal members 732 located between an extension base 736, and a topring or toroidal member 734.

The body 715, as shown in FIG. 7, includes hinges 733 on opposing faces741 and 743. Each of the faces 741 and 743 includes an indentation 740.Located within an interior portion of the body 715 is a spring member745. As shown in FIG. 7, the spring member 745 in this particularembodiment is a cantilevered spring, which can be made of metal or othermaterials, such as those described above. It can be seen from FIG. 7that the spring 745 can be selected with respect to its size, shape andmaterial composition so that the spring 745 exerts a force outward fromthe longitudinal axis of the fluidic connector 701 and against the faces741 and 743.

Still referring to FIG. 7, it can be seen that the lower portion of thebody 715 is located within a port in a body 770. The port in the body770 can be a port in any one of a number of components or fittings in anAI system. As shown in FIG. 7, the body 715 has projections 737 whichhave a greater outer diameter than the portions of the body 715 aboveand below the projections 737. The projections 737 hold the lowerportion of the body 715 within the port, since the port opening has adiameter less than that of the outer diameter of the projections 737when the body 715 is in a normal or rest position.

The fluidic connector 701 shown in FIG. 7 also includes a first ring760, a second rig 765, and a spring 720. The spring 720 in thisparticular embodiment and as shown in FIG. 7 is a coiled spring, whichcan be made of metal or other appropriate materials, such as thosediscussed above. The spring 720 can be selected as to its size, shape,and material composition so that it exerts a desired force against bothupwards and downwards. Because the projections 737 hold the body 715 inplace once they are located as shown in FIG. 7 with respect to the portof body 770, the body 715 is securely connected to the body 770. Inaddition, the spring 720 exerts a downward force, thereby pressingdownwards on the backup ring 775 which, in turn, presses downwardly onthe flange 725 of the tubing 705, thus keeping the end of the flange 725of the tubing 705 pressed firmly and sealingly against the passageway inthe body 770.

Referring now to FIG. 8, an enlarged cross-sectional view of a portionof the body 715 and certain components is provided. As shown at the topend of the body 715, a portion of the base 736 of the extension member710 is located within and proximal to a top end of the body 715. Hinges733 are located in the body 715, also proximal to its top end, but asshown in FIG. 8, the hinges 733 are located on opposing faces 741 and743 of the body 715.

Referring now to FIG. 8, an enlarged cross-sectional view of a portionof the body 715 and certain components is provided, with the body 715connected to a port of a body 770. As shown at the top end of the body715, a portion of the base 736 of the extension member 710 is locatedwithin and proximal to a top end of the body 715. Hinges 733 are locatedin the body 715, also proximal to its top end, but as shown in FIG. 8,the hinges 733 are located on opposing faces 741 and 743 of the body715. Hinges 733 are adapted to allow the faces 741 and 743 to moveinwards towards the longitudinal axis of the fluidic connector 701 whena force is exerted thereon, such as when the faces 741 and 743 arepressed by an operator. When such a force is exerted, such as when anoperator presses on the faces 741 and 743, and the faces move inwardly,the projections 737 also move inwardly towards the longitudinal axis.Thus, an operator can remove the fluidic connector 701 from the body770, and disconnect the fluidic connector 701, by pressing on the faces741 and 743 and pulling the fluidic connector 701 upwards and away fromthe port of the body 770. Because the tubing 705 extends throughpassageways in each of the other components of the fluidic connector 701as shown in FIG. 7, the entire fluidic connector 701 can be removed fromthe body 770 without any need to disassembly or remove any of thecomponents of the fluidic connector 701 from one another.

Still referring to FIG. 8, undercut portions 771 are provided by thebody 770 as part of the port of the body 770. The undercut portions 771are adapted to receive and hold the projections 737 of the body 715 whenthe lower portion of the body 715 is inserted into the port. Theundercut portions 771 retain the projections 737 and keep the fluidicconnector 701 connected to the body 770 once the projections 737 havebeen inserted sufficiently far enough into the port of the body 770. Thespring 745 is selected so that it exerts an outward force on the faces741 and 743 of the body 715 and thus also exerts an outward force on theprojections 737 of the body 715, thereby retaining the projections 737in place and retained by the undercut portions 771 of the port andthereby maintaining the fluidic connector 701 in a sealed connectionwith body 770.

By pressing faces 741 and 743 inwardly and inserting the bottom portionof assembly 701 into a port of the body 770, then releasing the pressureon faces 741 and 743, an operator can obtain a connection that is sealedand leak-proof, and provides sufficient force to keep the sealedconnection (even when a fluid under high pressures, such as from about200 psi to 5000 psi or so is flowing through the tubing 705) in place.An operator can make a desired connection in a LC or other analyticalinstrument system using the assembly 701 without the need for anytorque, such as on a nut, without the need for a threaded connection,and without the need for any additional tools, such as a wrench or thelike. Moreover, an operator can disconnect the assembly 701 from a portin the body 770 by pressing faces 741 and 743 inwardly and pulling onthe body 715 or other portion of the assembly 701 and easily removingthe assembly 701 from the port of the body 770, again without the neededfor any torque, without the need for disconnecting a threaded engagement(such as if a nut with threads is used), and without the need for anyadditional tools.

Referring now to FIGS. 9 and 10, another embodiment of a fittingconnector assembly is shown in a cut-away view. In FIG. 9, the fluidicconnector 901 includes a body having a head 910 at one end and a lowerportion 912 at an opposing end. As seen in FIG. 9, a tube 905 extendsfrom above the head 910 and through a passageway extending through thebody. The tubing 905 has a flange 925 at its bottom end, which extendsout from the lower portion 912 of the body. As shown in FIG. 9, the head910 can be generally rectangular in shape (although head 910 cangenerally be of other shapes, such as circular, elliptical, and thelike). A slot 904 is shown on one side of the head 910; a similar andopposing slot (not shown in FIG. 9) would be on the opposing side inthis particular embodiment, thus providing a generally rectangular head910 with four sides, two opposing sides of which have slots therein. Thefluidic connector 901 also includes a spring member 945 located at ornear the top end of the body 910. The head 910 also has splines 907,which are provided to allow an operator to grasp and manipulate thefluidic connector 901 easily. The fluidic connector 901 has an interiorportion, which includes a tapered portion 902, which is generallyconical in shape. As shown in FIG. 9, located below the head 910 areprojections 937 extending out from the longitudinal axis of the fluidicconnector 901. Located below the lower portion 912 of the body is aspring 920, which in this particular embodiment can be a coiled spring.FIG. 10 provides a cross-sectional view of the embodiment of the fluidicconnector 901 shown in FIG. 9.

An operator can easily assembly the fluidic connector 901 by placing thetubing 905 through the spring 920, through the central passagewaythrough the lower portion 912 and head 910 of the body, and through thecentral passageway through the spring member 945, and extending from thetop end of the fluidic connector 901. The spring member 945 can beremovably secured within the head 910 if desired, or can be permanentlyattached or secured to the head 910. The spring member 945 can be anyone of number of different types of spring members, such as anelastomeric spring. The spring member 945 serves to push or bias thefour sides of the head 910 outward from the longitudinal axis of thefluidic connector 901. However, the spring member 945 can be selected(such as by selecting its shape, size, and material composition) so thatan operator can easily push the two opposing sides of the head 910 whichdo not have slots 904 towards the longitudinal axis. The slots 904 allowthe other two sides to move towards each other, and thus the projections937 move towards each other and the longitudinal axis, thereby providinga smaller outer diameter than when in a resting or normal position (suchas when no additional force is applied to opposing sides of head 910).By squeezing the two opposing sides of head 910 which do not have slots904, the operator can move the projections closer together for easyinsertion of the lower portion of the fluidic connector 901 into a portand, when the operator releases the two opposing sides, the projections937 move outwardly from the longitudinal axis of the fluidic connector901 and, due to their now greater outer diameter, can be securely heldin place in the port. To remove the fluidic connector 901 once securedto a port, the operator can squeeze the two opposing sides of head 910which do not have slots 904 towards one another and towards thelongitudinal axis, thereby moving the projections 939 towards thelongitudinal axis and reducing the outer diameter of the two projections937, at which point the operator can easily pull the entire (and stillassembled) fluidic connector 901 from the port. The fluidic connector901 thus allows an operator to quickly connect the fluidic connector 901to a port, to quickly disconnect the fluidic connector 901 from a port,all without requiring the use of any tools or applying a particularforce beyond that necessary to insert the fluidic connector 901 into theport as described. Moreover, the spring 920 pushes the flange 925against the bottom of a port and thereby ensures a sealing connectionwhen the fluidic connector 901 is connected to a port as just described.

Referring now to FIG. 11, another embodiment of a fluidic connector 1101is shown. In FIG. 11, the fluidic connector 1101 includes an extensionportion 1110 and a body 1115. The extension 1110 includes a top member1134 and a series of toroidal members 1132. Also included as a part ofextension 1110 (but not shown in FIG. 11) is a spine member. The base1136 of the extension 1110 is located within a portion of the body 1115.The body 115 includes two opposing faces 1141 and 1143, each of whichhas an indentation 1140. In addition, each of the opposing faces 1141and 1143 has a hinge 1133 to allow at least a portion of the face 1141and 1143 to move inwardly and outwardly with respect to the longitudinalaxis of the fluidic connector 1101. The fluidic connector 1101 alsoincludes a spring 1145 located within an interior portion of the body1115. The body 1115 also includes a lower portion which has projections1137 adapted for removable engagement as described below. Located belowthe body 1115 is a ring member 1165, and below the ring member 1165 is aspring 1120, which in this particular embodiment and as shown in FIG. 11is a coiled spring. As can be seen from FIG. 11, the tubing 1105 has aflange 1125 at one end, and the tubing 1105 extends along thelongitudinal axis of the fluidic connector 1101 through a central openportion of the spring 1120 and through passageways in the ring 1165, thebody 1115, the base 1136, and the extension 1110.

In FIG. 11, the fluidic connector 1101 is connected via an adapter 1185to a port in a component or fitting 1170, such as a component or fitting1170 of the type used in an AI system. As shown in FIG. 11, theprojections 1137 have an outer diameter greater than the top opening ofthe adapter 1185. The undercut portions of the adapter 1185 hold theprojections 1137 securely in place relative to the adapter 1185 oncethey are inserted into the adapter 1185. As also shown in FIG. 11, theadapter 1185 has a portion which is externally threaded in thisembodiment. Moreover, the port of component 1170 is internally threaded,and the external threaded portion of the adapter 1185 is selected andadapted so that adapter 1185 can be screwed into the port of thecomponent 1170 and thereby securely and removably held in place andconnected to the component 1170. As the adapter 1185 is screwed into theport of component 1170, the spring 1120 in the fluidic connector 1101will be compressed and will exert a force upon the backup ring 1175,which in turn will exert a force on the flange 1125 and urge or pressthe flange 1125 against the bottom of the port of component 1170 asshown in FIG. 11.

Referring now for FIG. 12, an enlarged cross-sectional view of a portionof the connected fluidic connector 1101 shown in FIG. 11 is provided.Like numerals are used for the same features and components shown inboth FIGS. 11 and 12, although additional numerals are used for certaindetails better illustrated in FIG. 12. As shown in FIG. 12, the tubing1105 extends through a spring 1120, and both tubing 1105 and spring 1120are located within a passageway in an adapter 1185. The tubing 1105 hasa flange 1125 at a bottom end, which is pressed against the bottom ofthe port of the component 1170. As shown in FIG. 12, a fluid passageway1180 extends from the bottom of the port of component 1170. The fluidicconnector 1101 in FIG. 12 also shows the backup ring 1175 in moredetail. The backup ring 1175 is located between the bottom of the spring1120 and the top of the flange 1125. The backup ring 1175 at its bottomend or side has a smaller diameter than at its top end or side. Thebackup ring 1175 can be used to concentrate the force exerted on theflange 1125 to a smaller or reduced area of the surface of the flange1125, which has the advantage of keeping the fluid pressure area assmall as possible during operation when a fluid is flowing underpressure through tubing 1105. The backup ring 1175 thus helps maintain asealed connection between the fluidic connector 1101 and the component1170. As also shown in FIG. 12, the port of the component 1170 has alower portion 1203 and, above that, an internally threaded portion withinternal threads 1202. As noted above, the adapter 1185 has anexternally threaded portion with external threads 1201. The externalthreads 1201 are selected so that they can be removably and securelyengaged with the internal threads 1202 of the port of component 1170.

Referring now to FIG. 13, another embodiment of a fluidic connector isprovided. As shown in FIG. 13, a component 1370 has a tee connectionamong fluid passageways 1390, 1391, and 1392. In this particularembodiment, the tube 1305 of the fluidic connector does not have apassageway therethrough, but is solid. Alternatively, the tube 1305could be made of a semi-permeable material to allow some fluid to flowtherethrough, but only under pressure. As shown in FIG. 13, a bottom endof the tube 1305 is located adjacent to the bottom of the port 1308 ofthe component 1370 in the connected mode. Also shown in FIG. 13 is abottom sleeve 1303. The sleeve 1303 in this embodiment is attached tothe tube 1305. The sleeve 1303 can be attached to the tube 1305 throughtechniques such as fusing, crimping or overmolding. The sleeve 1303 canbe advantageous because it provides a surface which is biased againstthe bottom of the port by the spring 1320 and provides additionalsurface area to provide a seal.

In this particular embodiment in FIG. 13, the fluidic connection acts asa pressure relief, such that when the pressure of a fluid in passageways1390, 1391, and 1392 exceeds a certain threshold amount, the tube 1305will be pushed away from a sealing engagement with the bottom of theport and fluid will flow through passageway 1390 and into the port 1308.Those skilled in the art will appreciate that the force exerted on thering 1303 by the spring 1320 can be selected by selecting the shape,size and material composition of the spring 1320.

Referring now to FIGS. 14, 15, and 16, enlarged cross-sectional viewsare provided with respect to additional details of alternativeembodiments of a fluidic connector in accordance with the presentdisclosure. Like features and components have the same numerals in FIGS.14, 15, and 16 for convenience only; it will be apparent that each ofFIGS. 14, 15, and 16 illustrates a different potential embodiment inaccordance with the present disclosure.

Referring first to FIG. 14, a portion of a port 1408 in a component 1470is shown. Located within the port 1408 is the bottom portion of afluidic connector. In FIG. 14, the bottom end of tube 1405 is adjacentto the bottom of the port 1408 and sealingly engaged therewith. Thefluidic connector includes a spring 1420 located above a sleeve 1403. Asshown in FIG. 14, a passageway for fluid communication extends throughthe tube 1405 and is aligned with the passageway 1480 in the component1470. As shown in FIG. 14, the bottom end of the tube 1405 in a sealingengagement with the port 1408 is an essentially or substantially flatsurface.

Now referring to FIG. 15, a view of an embodiment similar to that shownin FIG. 14 is provided. In FIG. 15, a tube 1505 extends through thespring 1420 and sleeve 1403 and the fluidic connector is located andconnected to a port 1408 in a component 1470. The passageway in tube1505 is aligned with the passageway 1480 in component 1470. Instead of aflat surface at the bottom of the tube 1505, however, a portion of thetube with a smaller diameter (and thus a smaller or reduced surfacearea) is in contact with the bottom of the port 1408. The tube 1505 hasa reduced diameter portion 1501 at its bottom end, and a taperingportion 1502 slightly above the reduced diameter portion 1501. The useof a reduced diameter portion 1501 is helpful in concentrating thepressure load in a smaller area and thereby increases the pressure atthe seal area. This has the advantage of allowing the fluidic connectorto remain sealed when connected even at higher fluid pressures.

Another embodiment is shown in FIG. 16. In FIG. 16, a tube 1605 isconnected via a fluidic connector in a port 1408 in a component 1470.The passageway through the tube 1605 is aligned with the passageway 1480in the component 1470. As also shown in FIG. 16, a sleeve 1403 islocated above the bottom end of the tube 1605 and below the spring 1420.In FIG. 16, the bottom face of the tube 1605 has a reduced diameter andsmaller surface area 1601, with this reduced area 1601 providing thesealing engagement with the bottom of the port 1408. The tube 1605 has atapered portion 1602 between the reduced area portion 1601 and theportion of tube 1605 above it. As with the embodiment shown in FIG. 15,the embodiment shown in FIG. 16 provides the advantage of concentratingthe load in a reduced area and thereby allows operation with a sealedconnection at higher pressures.

Referring now to FIGS. 17-20, various views are provided of anotherembodiment of the present disclosure. In FIG. 17, a cross-sectional viewof a connection assembly 1701 as connected to a port in a body 1770 isprovided. As shown in FIG. 17, the assembly 1701 has tubing 1705extending along a longitudinal axis of the assembly 1701, an extensionmember 1710 having a top portion 1734, a series of toroidal members1732, and a base 1736. It will be appreciated that these variousfeatures correspond to the extension member 10, toroidal members 32, topmember 34, and base 36 described above and the foregoing description ofsuch corresponding features applies equally. In FIG. 17, a bottomportion of the assembly 1701 is located in a port of a body 1770, withthe bottom portion including a ring member 1765, a spring 1720, a tipmember 1750, and a ring 1755. As shown in FIG. 17, one end of the tubing1705 is adjacent to and abutting the bottom of the port, and portions ofthe tip member 1750 surrounding the tubing 1705 are also adjacent to andabutting the bottom of the port of the body 1770.

An enlarged cross-sectional view of the bottom portion of the assembly1701 shown in FIG. 17 is provided in FIG. 18. In FIG. 18, the tip member1750, the tubing 1705, and the ring 1755 are shown near the bottom ofthe port of the body 1770. Those skilled in the art will appreciatethat, as shown in FIG. 18, when the assembly 1701 is assembled andconnected to a port in a body 1770, the spring 1720 urges the tops ofthe ring 1755 and tip 1750 towards the bottom of the port of the body1770, thus providing a seal with sufficient force to prevent any leaksand prevent the tubing 1705 from being pushed out of the assembly 1701,even when fluids at high pressures, such as from about 200 psi or so toabout 5000 psi or so, are flowing through the tubing 1705 and theassembly 1701 as connected to the port of the body 1770 as shown in FIG.18. In addition, it will be appreciated that the spring 1720 pressesagainst and exerts an upward force on the ring member 1765, therebyurging the bottom of the body 1715 and the projections near the bottomof the body 1715 (not shown in FIG. 18) against the top of the port ofthe body 1770. FIG. 19 provides an additional view of the connectionassembly shown in FIGS. 17 and 18, with the assembly shown in a sealingengagement, and with like features having the same numbers forconvenience of the reader.

Referring now to FIG. 20, additional details are shown in the enlargedcross-sectional view of the fitting assembly 1701 as connected to a portin a body 1770. As shown in FIG. 20, the tip 1750 has a first end and atop end, wherein the top end has a portion 1752 which has a smallerdiameter than the bottom portion 1751. As shown in FIG. 20, the bottomportion 1751 provides a greater area for contact with the bottom of theport of the body 1770 (although such contact is not shown in FIG. 20).As also shown in FIG. 20, the ring 1755 has a top end and a bottom end,wherein the top end has a portion 1756 with an exposed surface areagreater than the surface area of the bottom end 1757 of the ring 1755.As can be seen in FIG. 20, each of the ring 1755 and the tip 1750 havetapered portions between their respective top and bottom ends as shownin FIG. 20. In the case of the ring 1755, the internal diameter definesa tapered portion, with the internal diameter at the bottom of the ring1755 greater than the internal diameter of the ring 1755 at its top. Inthe case of the tip 1750, the outside diameter of the tip 1750 defines atapered portion, with the bottom of the tapered portion of the tip 1750having a greater outside diameter than the top of the tapered portion ofthe tip 1750, all as shown in FIG. 20. It will be appreciated that, whenthe assembly 1701 is connected to the port of the body 1770 and thespring 1720 exerts a force urging the ring 1755 and the tip 1750 againstthe bottom of the port of the body 1770, the bottom end 1757 of the ring1755 will be urged towards the longitudinal axis of the assembly 1701and exert a force inwardly on the tip 1750 to prevent any leaks betweenthe tubing 1705 and the tip 1750.

The ring 1755 can be made of any one or more of the following materials,as may be desired for the anticipated use(s) of the assembly 1701: PEEK,PEAK, PEKK, PEI, and/or PPSU. The tip 1750 can be made of any of thefollowing materials, as may be desired for the anticipated use(s) of theassembly 1701: polyetheretherketone (PEEK), polyaryletherketone (PAEK),polyetherketoneketone (PEKK), fluorinated ethylene propylene (FEP),ethylene tetrafluoroethylene (ETFE), polytetrafluoroethylene (PTFE),perfluoroalkoxy (PFA, also called perfluoroalkoxyethylene),polychlorotrifluoroethylene (PCTFE), polymer-sheathed fused silica (suchas PEEKSi1), fused silica, or silica borite, and can further include afiller material, which can include fibers, such as carbon fibers, glassfibers, nanofibers, and/or metallic fiber, depending on the pressuresand fluids involved. Those skilled in the art will appreciate that thering 1755 and tip 1750 can be of any number of desired shapes and sizes,preferably so that the tapered portions of the ring 1755 and the tip1750 are adapted to cooperate with one another when the assembly 1701 isconnected to the port of the body 1770.

Referring now to FIGS. 21-25, another alternative embodiment of theconnection assembly in accordance with the present disclosure isprovided. As shown in FIG. 21, an assembly 2101 includes an extensionmember 2110, tubing 2105, a body 2115, a spring 2120, and a flange 2108at one end of the tubing 2105. From the prior discussion, it will beapparent that the fitting assembly 2101 is adapted to engage with aflat-bottomed port, such as in a component in an AI system (not shown inFIG. 21). The extension member 2110 in this particular embodiment isattached to the top 2136 of the body 2115. As shown in FIG. 21, the body2115 has four sides, which can be considered a front face, an opposingback face, and a side 2141 and an opposing side 2143 (not shown in FIG.21). As shown in FIG. 21, the side 2141 has a projection 2137, which isadapted to engage with a port and removably hold the assembly 2101 in asealing connection with a port.

Now referring to FIGS. 22 and 23, alternative views of the assembly 2101are provided. FIG. 22 provides a view of one face of the assembly 2101(which for discussion purposes can be referred to as the “front” face),and FIG. 23 provides a cross-sectional view of the assembly 2101 takenalong line E-E of FIG. 22. It will be appreciated that the same numbersare used to indicate the same features in FIGS. 21-25 for ease ofreference by the reader. As shown in FIG. 22, the body 2115 has twoopposing sides 2141 and 2143, each of which has projections 2141 a and2143 a, respectively. In addition, each of the two sides 2141 and 2143has lower portions which have projections 2137 on each side of the body2115. In the assembly shown in FIG. 22, an operator can push on theprojections 2141 a and 2143 a to push the two opposing sides 2141 and2143 of the body 2115 inwardly towards the longitudinal axis of theassembly 2101, thus moving projections 2137 inwardly as well anddecreasing the length between the tips of the two opposing projections2137. Doing so allows the assembly 2101 to be quickly and easilyinserted into, or removed from, a port (not shown in FIG. 22 or 23).

Referring now to FIGS. 24 and 25, additional alternative views of theassembly 2101 are provided. FIG. 24 provides a view of one side 2141 ofthe assembly 2101 (which for discussion purposes can be referred to as a“side” face), and FIG. 25 provides a cross-sectional view of theassembly 2101 taken along line F-F of FIG. 24. As noted, it will beappreciated that the same numbers are used to indicate the same featuresin FIGS. 21-25 for ease of reference by the reader. As shown in FIG. 24,the side 2141 of the body 2115 has two members 2135 a and 2135 b oneither side of the projection 2141 a. Moreover, the projection 2141 a isshown to have a generally rectangular or parallelogram shape, withrounded corners. Those skilled in the art will appreciate that the shapeof the projection 2141 a can easily be varied as may be desired. Forexample, the shape shown in FIGS. 21-25 provides a useful shape which iseasily used by an operator to push sides 2141 and 2143 towards oneanother, but other shapes can likewise be used, such as ellipses,circles, squares, triangles, and so forth.

Among other features to note include the alternating grooves in theextension member 2110. As indicated by a comparison of FIGS. 21-25, itcan be seen that the grooves in extension member 2110 are essentiallyarcs which extend roughly 180 degrees around the generally conical edgeof the extension member 2110. Again, those skilled in the art willappreciate that differing patterns and configurations can be used forthe extension member 2110. The alternating grooves in extension 2110provide a balance between the strength needed for the extension 2110 toprovide effective stress relief for the tubing 2105, while avoiding theuse of too much material that is not needed. Those skilled in the artwill appreciate that the features of the assembly 2101 as shown in FIGS.21-25 can be made of the same materials as similar items and features inthe embodiments previously described in this disclosure.

Additional embodiments of a quick connect/disconnect assembly areillustrated in FIGS. 26-31. It will be appreciated that like featuresand items will have the same numbering in FIGS. 26-31 for convenience.In FIG. 26, a cross-sectional view of an assembly 2601 is provided whichincludes a body 2602 which has a passageway therethrough in which tubingis located along the longitudinal axis of body 2602, with the tubinghaving an inner tube 2605 b and an outer tube 2605 a. Also locatedwithin a first portion of the body 2602 (i.e., the “top” portion of body2602 in FIG. 26) is a spring element 2620. As illustrated in FIG. 26,the spring 2620 is a coiled spring which is located around the tubingand within the body 2620. Also located within the body 2602 is a bearingelement 2625. As shown in FIG. 26, one side of the bearing element islocated at the bottom end of and abuts the spring 2620. The other sideof the bearing 2625 is adjacent to and abuts a sleeve 2610 whichsurrounds a portion of the outer tubing 2605 a (and thus inner tubing2605 b as well). The bottom portion of the body 2602 has inwardlyprojecting shoulders 2637, shown on both sides of the body 2602 in FIG.26, each having a face 2638.

The assembly 2601 in FIG. 26 also includes a port 2670, which can be aport for an AI system or for any component in an AI system. The port2670 has an opening at one end through which the tubing 2605 a and 2605b extends. The port 2670 also has a flat-bottomed port and a passageway2675 which allows for fluid communication with a fluid in the interiorof the inner tube 2605 b. The port 2670 also includes projections 2677on both sides, each of which has a projection face 2678. As shown inFIG. 26, the body 2602 and port 2670 are removably attached to eachother and held together by the engagement of the projections 2677 of theport 2670 and the shoulders 2637 of the body 2602, with the each of thefaces 2678 abutting the corresponding faces 2638. The assembly 2601 isthus shown in a connected configuration in FIG. 26.

The inner and outer tubing 2605 a and 2605 b are configured so that oneend of the inner tubing 2605 b abuts against the bottom of theflat-bottomed port of port body 2670. Also shown in FIG. 26 is a sleeve2610, which surrounds a portion of the outer tubing 2605 a. One end ofthe sleeve 2610 abuts one side of the bearing 2625, and the other end ofthe sleeve 2610 has a recess therein in which a tip 2615 is located. Theconfiguration and use of the tubing with sleeve 2610 and tip 2615 isdescribed in more detail in co-pending U.S. non-provisional patentapplication Ser. No. 14/992,041, filed Oct. 23, 2015 and titled“Face-Sealing Fluidic Connection System,” which is hereby incorporatedby reference as if fully set forth herein. Those skilled in the art willappreciate and understand, however, that the present disclosure and itsembodiments may such use other tubing configurations as may be desirablefor a given application, and are not limited to those shown anddescribed herein.

Those skilled in the art will appreciate that, depending on the intendedapplication, including without limitation the expected fluidicpressures, the nature of the fluids to be used, the nature of thesamples to be analyzed, and the like, the composition of the materialsused, as well as the specific shapes and sizes, of the variouscomponents and features of the fluidic connector assembly 2601 as shownand described above can be varied. For example, in applications in whichbiocompatibility is desired, the tubing 2605 a and 2605 b can be made ofthe same or different materials, including for example[polyetheretherketone (PEEK), polyaryletherketone (PAEK),polyetherketoneketone (PEKK), fluorinated ethylene propylene (FEP),ethylene tetrafluoroethylene (ETFE), polytetrafluoroethylene (PTFE),perfluoroalkoxy (PFA, also called perfluoroalkoxyethylene),polychlorotrifluoroethylene (PCTFE), polymer-sheathed fused silica (suchas PEEKSil), fused silica, or silica borite, and can further include afiller material, which can include fibers, such as carbon fibers, glassfibers, nanofibers, and/or metallic fibers, depending on the pressuresand fluids involved. It is anticipated that the assembly 2601 will be ofparticular usefulness in applications in which biological materials areinvolved. In such situations, it is often desirable for the fluid pathto contain only biocompatible materials. In the embodiment shown anddescribed, this is achieved because the tubing 2605 b, the tip 2615 andthe port body 2670 are the only components of the assembly 2601 touchingthe fluid, an even then tip 2615 need not necessarily come into contactwith the fluid if the sealing connection between the end of the innertubing 2605 b and the port body 2670 is made. In the embodiment shown inFIG. 26, then, the spring 2620, as well as the body 2602, the bearing2625, and the sleeve 2610 can be made of metal, such as stainless steel,steel, titanium, nickel, and/or nitinol. Alternatively, the spring 2620,as well as the body 2602, the bearing 2625, and the sleeve 2610 can bemade of metal, or can be made of PEEK, PEAK, PEKK, PEI, and/or PPSU.

A user or operator can quickly and easily connect the body 2602 and port2670. To connect the body 2602 and port 2670, a user can insert one endof tubing 2605 a and 2605 b into and through the passageway through thelongitudinal axis of body 2602, through the sleeve 2610, and into theopening of port 2610. The user can then pinch the two tabs 2640extending radially outwardly from the longitudinal axis of the body2602, and push the two tabs 2640 towards the longintudinal axis (i.e.,radially inwardly). By doing so, the user thus moves the two inwardlyprojections 2637 of the body radially outwardly, and thereby increasesthe inner diameter between the two projections 2637. Once the innerdiameter between the two projections 2637 is greater than the outerdiameter of the two shoulders or projections 2677 of the port 2670, thebottom end of the body 2602 can easily and quickly fit over theprojections 2677. Once the projections 2637 are below and past theprojections 2677, the user can simply release the force on the two tabs2640 and the projections 2637 of body 2602 will automatically move backradially inwardly, thereby engaging faces 2638 and 2678 so that the body2602 and the port 2670 are held securely and removably together. Todisconnect the body 2602 and port 2670 from one another, a user cansimply urge the tabs 2640 radially inwardly and towards the longitudinalaxis of the assembly 2601, thereby moving the projections 2637 of thebody 2602 radially outward and disengaging the projections 2637 and2677. Once the inner diameter defined by the two projections 2637 isgreater than the outer diameter defined by the projections 2677 of theport 2670, the body 2602 can be pulled away and removed from the port2670 quickly and easily. As shown in FIG. 26, the assembly 2601 is in aconnected configuration.

In this particular embodiment, the two tabs 2640 and the bottom portionof the body 2602 can be substantially rectangular in shape. Thoseskilled in the art will appreciate, however, that the tabs 2640 and/orbottom portion of the body 2602 can be curved and describe an arc aroundthe longitudinal axis, or can even be circular in shape and extend allthe way around the longitudinal axis of the assembly 2601 if desired.

Those skilled in the art will appreciate that, when making a connection,a user will also need to push the body 2602 towards the port 2670 andalong the longitudinal axis of the assembly 2601, and doing so willthereby compress the spring 2620. As the spring 2620 is compressed, itwill exert a force longitudinally against the bearing 2625 and therebyagainst the sleeve 2610, which in turn exerts a force against the tip2615, urging it against the bottom face 2673 of the port 2670 andsealing the inner tubing 2605 b in the port to provide a leak-free seal.The spring 2620 can be chosen so that its size and compressive force issuch that it fits within the body 2602 as shown and also is easilycompressed by a user by hand without the need for tools or fixtures, yetwill continue to exert a compressive force against the bearing 2625 tokeep the tip 2615 abutting the bottom of the port and thus keep theleak-free seal while the assembly 2601 remains in a connectedconfiguration. We believe that, with the appropriate selection ofmaterials and spring size, a leak-free seal of capillary tubing can beobtained in a flat-bottomed port with fluid flowing through the tubingat pressures of up to 20,000 psi without any leakage or extrusion of thetubing from the assembly 2601.

Referring now to FIG. 27, another cross-sectional view of the assembly2601 is provided. It will be appreciated that in FIG. 27, the body 2602,tubing 2605 a and 2605 b, spring 2620, and bearing 2625, as well assleeve 2610 and tip 2615 can be the same as shown in FIG. 26 and asdescribed above. In FIG. 27, the port 2670′ differs in that it no longerprovides a conventional flat-bottomed port (as was shown in FIG. 26 anddescribed above). Instead, the bottom of the opening adapted to receiveand sealingly hold the tubing in the port 2670′ has a standing bossportion 2672 which extends upwardly from the bottom of the opening andradially inwardly towards the longitudinal axis of the port 2670′, thusproviding a flat-bottomed face 2673′ which has a smaller diameter thanthe inner diameter of the opening of the port 2670′ into which thetubing is inserted and is received. As shown in FIG. 27, only the innertube 2605 b is in contact with the face 2673′ of the port 2670′. Thoseskilled in the art will appreciate, however, that the size of the face2673′ of the port 2670′ can be chosen so that, when desired, some or allof the tip 2615 and inner tube 2605 b together abut the face 2673′instead of just the tube 2605 b.

Conventional designs for ports and connections in most UHPLC and HPLCinstruments and components typically have a 10-32 coned port with a flatbottom for creating the fluidic seal with one end of tubing connectedtherein. The standing boss 2672 provides a feature that allows for useof the assembly 2601 with ports like port 2670′ in applications withelevated pressures not common for conventional quick connections.Conventional 10-32 port bottoms typically have a sealing area of 0.0032square inches, whereas the standing boss 2672 can be used to provide aport bottom face 2673′ with an area of 0.00031 square inches. Thesealing area becomes important when considering the pressure applied tothe end of the tubing. Assuming the tubing can be pressed by the user,via a fitting assembly 2601 or otherwise by conventional assemblies, toabout 10 lbs of load, then the pressure at the tube end in a standardport is 3,125 psi vs. 32,258 in a port 2670′ with the standing boss 2672and the reduced flat bottomed port face 2673′. Those skilled in the artwill appreciate that the standing boss 2672 can be provided in a numberof ways; it can be machined directly in the port bottom or can beobtained via the use of an additional insert that fits in the portbottom, or in other ways.

Now referring to FIG. 28, a cross-sectional view of an embodiment of aconnection assembly 2801 is provided. In FIG. 28, a body 2602 isprovided, which may be the same as the body 2602 described above. Thebody 2602 has tubing 2605 a and 2605 b extending through the passagewaythrough the body 2602, and has a coil spring 2620 located within a topportion of the body 2602 and around the tubing, with one end of the coilspring 2620 abutting an interior top end of the body 2602 and the otherend abutting one side of a bearing 2625. The body 2602 further has twotabs 2640 and radially extending inward projections 2677.

In FIG. 28, the assembly 2801 includes one end of a column 2855, whichhas an exterior or outer layer 2850. One end of the outer layer 2850 hasexternal threads 2851, which are adapted to securely engage with theinternal threads 2861 of the adapter 2860. As shown in FIG. 28, theadapter 2860 has a first end which provides a port adapted to securelyand sealingly receive one end of tubing 2605 a and 2605 b, as well assecurely and removably engage with the body 2602 via the projections2877 on either side of the adapter 2860. The other end of the adapter2860 (i.e., the bottom end as illustrated in FIG. 28) has a recesstherein which has an internally threaded portion 2861. As shown in FIG.28, a first portion of the recess in the bottom end of the adapter 2860is adapted to removably engage with the outer layer 2850 and column2855.

The adapter 2860 also has a seat portion 2862 therein which, in theembodiment shown in FIG. 28, has an insert 2870 removably seatedtherein. As shown in FIG. 28, the insert 2870 has a first side which hasstanding boss 2872 which provides a face 2873 against which the innertube 2605 b and at least a portion of the tip 2615 abut to provide aremovably sealing engagement. The standing boss 2872 provides the sameadvantages as described above with respect to a smaller area for formingthe seal with one end of the tubing. The second side of the insert 2870has a recess portion, in which a filter element 2880 (such as a frit) islocated. Those skilled in the art will appreciate that, although FIG. 28illustrates the use of the body 2602 with an adapter 2860 for connectingto one end of a column 2855, the adapter 2860 can be used with body 2602to provide a quick and easy connect/disconnect function (such asdescribed above) in connection with any one of a number of components inan LC or AI system.

Turning now to FIG. 29, a cross-sectional view of an assembly 2901 isillustrated to show another embodiment of this disclosure. The assembly2901 includes a body 2602 through which one end or tubing 2605 a and2605 b extends, together with a spring 2620 located within and along thelongitudinal axis of the body 2602. The body 2602 has tabs 2640 andradially inward projections 2637 as described above.

In the assembly 2901, an adapter 2970 is used together with the body2602 to connect the tubing 2605 a and 2605 b to a union 2910. Asillustrated in FIG. 29, the union 2910 may have a conventional port,such as one having an internally threaded portion with 10-32 threads, aconical taper, and then a flat-bottomed face for sealing against an endof a tube. As shown in FIG. 29, the adapter 2970 has a passagewaytherethrough along its longitudinal axis, with a first or top end asshown in FIG. 29 having proximal thereto radially outwardly extendingshoulders or projections 2977 on either side, each of which have faces2978 and are adapted to removably engage with the projections 2637 oneither side of the body 2602. In addition, the first or top end of theadapter 2970 has an opening therein which is adapted to receive one endof the tubing 2605 a and 2605 b, together with the sleeve 2610.

The bottom end of the adapter 2970 has an externally threaded portion2951, which is adapted to removably engage with an internally threadedrecess portion of the first or top end of the union 2910 shown in FIG.29. As shown in FIG. 29, the union 2910 may have two ends, each havingan internal port, each with an internally threaded portion, a conicallytapered portion, and a flat-bottomed portion for receiving one end of atube or, as shown in FIG. 29, a connector 2920. The connector 2920 shownin FIG. 29 has two ends and a fluid passageway extending therethroughalong its longitudinal axis. The first or top end of the connector 2920has a portion which is of a size and shape that is adapted to removablyfit within passageway 2972 of the adapter 2970. The adapter 2970 has aneck portion 2921, which has a narrower inner diameter than the portionof the passageway 2972 below the neck portion 2921, as well as theportion of the passageway 2972 located above the neck portion 2921 asillustrated in FIG. 29. The first or top end of the connector 2920extends through the passageway 2972 of the adapted 2970 and past the topend of the neck portion 2921. As shown in FIG. 29, the first or top endof the connector 2920 abuts against the bottom end of the tip 2615 andthe inner tube 2605 b to provide a removable seal, with the passagewaysin the inner tube 2605 b and the connector 2920 aligned to allow fluidto pass therethrough when the assembly 2901 is connected as is shown inFIG. 29.

A cross-sectional view of another alternative embodiment is provided inFIG. 30. A connection assembly 3001 (shown in a connected configuration)includes a first body 3002, a second body 3003, a spring element 3020, awasher element 3025, and a bearing element 3068, each having apassageway therethrough along a longitudinal axis of the assembly 3001.Located within the passageway of each of the first body 3002, secondbody 3003, spring 3020, washer 3025, and bearing 3068 is tubing 3005. Asshown in FIG. 30, one end of tubing 3005 extends through the passagewayof the assembly 3001 and a portion of which is located in a first portat one end of a connector 3070. Also shown in FIG. 30 is a tip 3015 anda sleeve 3010, each of which surrounds a portion of the tubing 3005.

The first body 3002 has arms or tabs 3040 on either side. It will beappreciated that the arms 3040 can be rectangular, arc-shaped, orcircular and extend all the way around the longitudinal axis of the body3002, or may have such other shape as desired. The body 3002 also hasinwardly radially projecting shoulders 3006 on either side. When a userpresses the arms 3040 radially inwardly, the shoulders 3006 also moveradially inwardly. When the shoulders 3006 move radially inwardly, theypress inwardly against portions 3007 of the second body 3003. When theportions 3007 of the second body 3003 are moved inwardly towards thelongitudinal axis of the assembly 3001, the projections 3077 at thesecond or bottom end of the second body 3003 also move radially inwardlyand therefore define a narrower outer diameter proximal the second orbottom end of the second body 3003. As shown in FIG. 30, the projections3077 in a connected configuration are located and removably held withina recess 3040 proximal a first or top end of the connector 3070. Inaddition, a bearing 3067 is located on the bottom side of the secondbody 3003 and a first or top side of the bearing 3067 abuts against thebottom end of the second body 3003.

As also shown in FIG. 30, located within the passageway of the secondbody 3003 and proximal a first or top end thereof is a spring element3020. A top side of the spring 3020 abuts against an interior surface ofthe top end of the second body 3003, while the bottom side of the spring3020 abuts against a first or top side of the washer 3025. The first ortop side of the washer 3025 has shoulders adapted to receive and holdthe bottom side of the spring 3020, while the bottom side of the washerhas shoulders adapted to receive and hole a first or top end of thesleeve 3010.

The connector 3070 in FIG. 30 has at a top or first end an openingdefined by radially inward projections 3071, which define an innerdiameter narrower than that of the recess 3040. As also shown in FIG.30, the connector 3070 has a flat-bottomed port at one end which isadapted to receive and sealingly hold one end of the tubing 3005, tip3015, and sleeve 3010. The connector 3070 further has a passageway 3075from the flat-bottomed port to a second port, which as shown in FIG. 30,may be of a conventional type of port, with a flat bottom for receivingan end of tubing 3090 therein, a conically tapered portion for engagingwith a ferrule 3091, and an internally threaded portion adapted toremovably engage with an externally threaded end of a nut 3092.

Those skilled in the art will appreciate that, depending on the intendedapplication, including without limitation the expected fluidicpressures, the nature of the fluids to be used, the nature of thesamples to be analyzed, and the like, the composition of the materialsused, as well as the specific shapes and sizes, of the variouscomponents and features of the fluidic connector assembly 3001 as shownand described above can be varied. For example, in applications in whichbiocompatibility is desired, the tubing 3005 can be made of the same ordifferent materials, including for example [polyetheretherketone (PEEK),polyaryletherketone (PAEK), polyetherketoneketone (PEKK), fluorinatedethylene propylene (FEP), ethylene tetrafluoroethylene (ETFE),polytetrafluoroethylene (PTFE), perfluoroalkoxy (PFA, also calledperfluoroalkoxyethylene), polychlorotrifluoroethylene (PCTFE),polymer-sheathed fused silica (such as PEEKSi1), fused silica, or silicaborite, and can further include a filler material, which can includefibers, such as carbon fibers, glass fibers, nanofibers, and/or metallicfibers, depending on the pressures and fluids involved. It isanticipated that the assembly 2601 will be of particular usefulness inapplications in which biological materials are involved. In suchsituations, it is often desirable for the fluid path to contain onlybiocompatible materials. In the embodiment shown and described, this isachieved because the tubing 3005, the tip 3015 and the port body 3070are the only components of the assembly 3001 touching the fluid, andeven then tip 3015 need not necessarily come into contact with the fluidif the sealing connection between the end of the tubing 3005 and theport body 3070 is made.

In the embodiment shown in FIG. 30, then, the spring 3020, as well asthe first body 3002, the second body 3003, the bearing 3025, the washer3068, and the sleeve 3010 can be made of metal, such as stainless steel,steel, titanium, nickel, and/or nitinol. Alternatively, the spring 3020,as well as the first body 3002, the second body 3003, the bearing 3025,the washer 3068, and the sleeve 3010 can be made of one or morenon-metals, such as PEEK, PEAK, PEKK, PEI, and/or PPSU.

A user or operator can quickly and easily connect the assembly 3001. Toconnect the first body 3002 and second body 3003 with the connector3070, a user can insert one end of tubing 3005 into and through thepassageway through the longitudinal axis of first body 3002, second body3003, spring 3020, washer 3025, bearing 3068, and through the sleeve3010, and into the opening of connector 3079. The user can then pinchthe two tabs 3040 extending radially inwardly towards the longitudinalaxis of the body 2602, which then pushes the two shoulders 3006 towardsthe longitudinal axis (i.e., radially inwardly). By doing so, the userthus moves the two projections 3077 of the second body 3003 radiallyinwardly, and thereby decreases the outer diameter of the bottom end ofthe second body 3003 as defined by the two projections 3077. Once theouter diameter defined by the two projections 3077 is less than theinner diameter of the two shoulders or projections 3071 at the top endof the connector 3070, the bottom end of the second body 3003 can easilyand quickly fit past the projections 3071 and into the recess at the topend of the connector 3070. Once the projections 3077 are below and pastthe projections 3071, the user can simply release the force on the twotabs 3040 and the projections 3077 of the second body 3003 willautomatically move back radially outwardly, thereby engaging theprojections 3071 and 3077 so that the bottom end of the second body 3003and the top end of the connector 3070 are held securely and removablytogether. To disconnect the second body 3003 and the connector 3070 fromone another, a user can simply urge the tabs 3040 radially inwardly andtowards the longitudinal axis of the assembly 3001, thereby moving theprojections 3077 of the second body 3003 radially inward and disengagingthe projections 3077 and 3071. Once the outer diameter defined by thetwo projections 3077 is less than the inner diameter defined by theprojections 3071 of the connector 3070, the bottom end of the secondbody 3003 can be pulled away and removed from the connector 3070 quicklyand easily.

Those skilled in the art will appreciate that, when making a connection,a user will also need to push the first body 3002, and thereby thesecond body 3003, longitudinally towards the connector 3070 and alongthe longitudinal axis of the assembly 3001. Doing so will therebycompress the spring 3020. As the spring 3020 is compressed, it willexert a force longitudinally against the washer 3025 and thereby againstthe sleeve 3010, which in turn exerts a force against the tip 3015,urging it against the bottom face of the port at the top end of theconnector 3070 and sealing the tubing 3005 in the port of the connector3070 to provide a leak-free seal. It will be appreciated that the spring3020 can be selected so that it is strong enough to continue to exertenough force on the sleeve 3010 and thereby tip 3015 as long as theassembly 3001 remains in a connected configuration, yet will be of suchstrength that a user will be able to compress the spring 3020 whenmaking a connection with assembly 3001 by hand and without the need forany tools or fixtures. We believe that, with the appropriate selectionof materials and spring size, a leak-free seal of capillary tubing canbe obtained in a flat-bottomed port with fluid flowing through thetubing at pressures of up to 20,000 psi without any leakage or extrusionof the tubing from the assembly 3001.

Referring now to FIG. 31, a cross-sectional view of another embodimentof a connection assembly is provided. In FIG. 31, the assembly 3101includes a first body 3002, a second body 3003, a spring 3020, a washer3025, tubing 3005, a tip 3015, a bearing 3068, and a sleeve 3010, eachof which may be configured like that described above, and each of whichhas passageways therethrough. It will be appreciated that the assembly3101 is shown in a connected configuration.

In FIG. 31, the assembly 3101 includes an adapter 3170, which can beused to connect one end of the tubing 3005 to a first end of a column3195. The adapter 3170 has a first or top end which has radially inwardprojections 3171, which are adapted to removably engage with theprojections 3077 of the second body 3003. As shown in FIG. 31, in aconnected configuration, the inner diameter defined by the projections3171 of the adapter 3170 is less than the outer diameter defined by theprojections 3077, so the bottom end of the second body 3003 is heldwithin the first or top end of the adapter 3170. The adapter 3170 alsoincludes an interior recess 3173 extending along a portion of itslongitudinal axis, with the recess 3173 of a shape and size andconfigured to receive and removably hold therein the sleeve 3010, tip3015, and tubing 3005. The bottom end of the recess 3173 defines aflat-bottom face.

An insert 3180 can be located at the top end of the recess defined atthe second or bottom end of the adapter 3170. As illustrated in FIG. 31,the insert 3180 has a first or top face and a second or bottom face.Because the top end of the insert 3180 has a standing boss 3182, the topface 3183 of the insert 3180 defines a smaller surface area than that ofthe port or recess 3173. The smaller surface area of face 3183 providesthe same advantages as described above by concentrating the pressureload applied by a user to connect the assembly 3101 and thereby allowthe assembly 3101 to operate leak-free at higher pressures.

Located in a recess on the bottom side of the insert 3180 is a filterelement 3190, which can be a frit of a conventional type. As shown inFIG. 31, the outer layer 3197 of the column 3195 is secured to thebottom end of the adapter 3170 by the mating of the external threadsproximal the top or first end of the outer layer 3197 and the internalthreads proximal the second or bottom end of the adapter 3170.

Referring now to FIGS. 32 and 33, an alternative embodiment of a quickconnect/disconnect assembly is illustrated. FIG. 32 is an explodedisometric illustration of an embodiment of an assembly in accordancewith the present disclosure in a disconnected configuration, while FIG.33 is a cross-sectional view of the assembly of FIG. 32 in a connectedconfiguration. In FIG. 32, the assembly 3201 provides an alternativeembodiment for a connection assembly which may be quickly and easilyconnected or disconnected by an operator or user by hand with an axialforce and without any requirement for any torque forces, tools, or otherequipment. The assembly 3201 includes a column 3210 which has attachedby threaded engagement at one end thereof an adaptor 3270, with theadaptor 3270 having a projecting end portion 3271. Also shown in FIG. 32is a portion of tubing 3205, which has at one end a tip 3215. A portionof the tubing 3205 extends through and along the longitudinal axis of aconnector 3280. Also shown in FIG. 32 is a collar 3202, which isproximal one end of the connector 3280 and extends around an exteriorportion of the connector 3280 at that end. It will be appreciated thatthe column 3210, adaptor 3270, connector 3280, and collar 3202 can bemade of a variety of materials, including stainless steel, steel,titanium, nickel, PEEK, PEAK, PEKK, PEI, and/or PPSU.

As shown in FIG. 33, the assembly 3201 is illustrated in across-sectional view in a connected configuration. The adaptor 3270 hasproximal one end an interior threaded portion adapted to removablyengage with an exterior threaded portion proximal one end of the column3210. As shown in FIG. 33, a frit or filter 3262 is located in a holder3260, with one side of the holder 3260 abutting against one end of thecolumn 3210. In addition, the holder 3260 has a tapered portion 3261 onthe side opposite the column 3210, with this latter side adapted to forma sealing engagement with the tip 3215 at one end of the tubing 3205.The tapered portion 3215 can be a frusto-conical shape or any othershape that provides a reduced diameter surface at the side of the holder3260 adapted to form a seal with the end of the tubing 3205 and/or tip3215 (as shown in this particular embodiment of FIG. 33).

The adapter 3270 has an end portion 3271 proximal the second end of theadapter 3270, which is located in a recess located within and proximalto a first end of the connector 3280. As shown in FIG. 33, the adapter3270 has a passageway therethrough, portions of which are adapted toreceive and hold the tubing 3205, a tubing sleeve 3210, as well as theholder 3260 and the first end of the column 3270. In addition, theadapter 3270 has a reduced outer diameter portion 3276 and, on eitherside thereof, has shoulder (or enlarged diameter) portions 3273 a and3273 b. As shown in FIG. 33, a ball 3250 is located between the shoulderportions 3273 a and 3273 b and rests against the exterior or the adapter3270 at the reduced diameter portion 3276. The ball 3250 and the adapter3270 thus form a ball detent connection to hold the collar 3202 andconnector 3280 in place once the ball 3250 is located between the twoshoulder portions 3273 a and 3273 b.

Still referring to FIG. 33, a spring 3221 is located in a gap arealocated between the interior surface of a portion of the collar 3202 andan exterior surface portion of the connector 3280, with one end of thespring 3221 abutting against a shoulder or radially outward projectingportion 3277 of the connector 3280. The spring 3221 serves to bias orpush the connector 3280 and collar 3202 away from one another, therebykeeping the collar 3202 in place proximal one end of the connector 3280unless and until an operator or user pushes the collar 3202 away fromthe column and towards the right in FIG. 33. Once an operator so pushesthe collar 3202 with enough force, the collar 3202 and ball 3250 willmove to the right of the shoulder 3273 a, and at that point the adapter3270 can be quickly and easily removed from the connector 3280 by simplypulling the two apart. The collar 3202 includes an enlarged diameterportion, or shoulder portion, 3206 in order to allow an operator toeasily grasp or push or pull the collar 3202 in whatever direction isdesired.

To connect the adapter 3270 and connector 3280 when they are not yetconnected, an operator can easily and quickly move the collar 3202 awayfrom the left end of the connector 3280 and towards the right end,thereby moving the ball 3250 up and out of the reduced diameter portion3276, then insert the first end portion 3271 of the adapter 3270 intothe recess in the end of the connector 3280. The operator can then pushthe collar 3202 back towards the left (in FIG. 33) and the ball 3250will engage and be located within the shoulders 3273 a and 3273 b. Theball 3250 and detent configuration and the spring 3221 will hold thecollar 3202 in place once an operator has made a connection. At one endof the collar 3202 are radially inwardly projecting shoulders 3204. Asshown in FIG. 33, the shoulder 3204 have an interior diameter which isless than that of the shoulders 3277 of the connector 3280, and lessthan that of the portion 3284 of the connector 3280. The shoulders 3277and 3284 thus prevent the collar 3202 from moving either left or rightby more than a specific amount. By selecting the distance between theshoulders 3277 and 3284, and by selecting the spring 3221, a preselectedbiasing force can be obtained to bias the collar 3202 and the connector3280 towards a position in which the collar 3202 is located at or nearthe end of the connector 3280 which abuts or is proximal the adapter3270.

The connector 3280 shown in FIG. 33 has a second spring 3220 locatedtherein. The spring 3220 is located in an interior passageway portion ofthe connector 3280, with one end held in the passageway by the end ofthe connector 3280, and the other end of the spring 3220 abutting oneside of a washer 3225. The other side of the washer 3225 abuts thesleeve 3210. The spring 3220 thus provides an axial force biasing thewasher 3225 and therefore the sleeve 3210, as well as the tubing 3205and the tip 3215, towards and against one side of the holder 3260. Asdetailed above, the reduced surface area of the holder 3260 which isprovided by the tapered portion 3261 allows a much greater force perunit of area to be obtained where the tip 315 abuts the holder 3261given a particular axial load from the spring 3220. The spring 3220 canbe selected so that it provides a preselected force to bias or push thetip 3215 against the holder 3261 to obtain a sealing engagement. It willbe appreciated that, depending on the intended application of theconnection assembly 3201, a higher or lower fluidic pressure may be usedand so a higher or lower pressure may be desired to ensure that thetubing 3205 is not extruded from the assembly 3201 by the fluidic forceand that there is no leakage of the fluid.

Referring now to FIGS. 34 and 35, another embodiment of a connectionassembly is provided. FIG. 34 is an exploded isometric view of theconnection assembly 3501 in a disconnected configuration, while FIG. 35is a cross-sectional view of the assembly 3501 in a connectedconfiguration.

In FIG. 34, the assembly 3501 includes a column 3510, an adapter 3570, acollar 3502, a connector 3580, and tubing 3505. As shown in FIG. 34, oneend of the tubing has a tip 3515. In addition, the collar 3502 has afirst or front portion 3503 b and a second or rear portion 3503 a. Inthis particular embodiment, the first portion 3503 b has on one sidethereof a button portion 3512. It will be appreciated that column 3510,adapter 3570, collar 3502, connector 3580, and tubing 3505 can comprisebiocompatible materials, such as PEEK or PEAK, PEKK, PEI, and/or PPSU,or can comprise other materials, such as stainless steel, nickel, steel,and/or titanium. As with the other embodiments described herein, thevarious items and features described and shown may comprise variousmaterials which may be selected depending on the application to whichthe assembly 3501 is intended for, such as applications involvingdifferent pressures and/or different fluids of various pH levels,corrosivity, and the like.

Referring now to FIG. 35, the assembly 3501 is shown in a connectedconfiguration in a cross-sectional view. As those skilled in the artwill appreciate, the column 3510, frit or filter 3562, and holder 3560with a tapered side 3561 may be like those shown and described elsewherein this disclosure. As shown in FIG. 35, the adapter 3570 has at one endan internally threaded portion with threads adapted to engage with anexternally threaded portion of one end of the column 3510. A first endof the connector 3580 abuts against portions of the adapter 3570. Theconnector 3580 includes a button portion 3512 on one side thereof, whichis connected to a latching portion 3503 b. The first latching portion3503 b is located within a recess on an exterior portion of theconnector 3580 and also within a groove 3583 in an exterior portion ofthe adapter 3570. In addition, the connection has a second latchingportion 3503 a, which is located within a second groove in the exteriorof the connector 3580.

To disconnect the assembly 3501 from the connected configuration shownin FIG. 35, an operator can push on the button portion 3512, therebypushing the first latching portion 3503 b out of the grooves 3583 of theadapter 3570, at which point the adapter 3570 and connector 3580 can bepulled apart from one another. From a disconnected configuration, theoperator can connect the assembly 3501 by pushing the button portion3512 radially inward and then inserting the adapter into the recess atone end of the connector 3580, then releasing the button 3512 andallowing the first latching portion 3503 b to return to its locationwithin the groove 3583 of the adapter 3570, thereby retaining theadapter 3570 and the connector 3580 in a connected configuration.

As also shown in FIG. 35, the connector 3580 has a passagewaytherethrough which is adapted to receive and hold the tubing 3505, aswell as a spring 3520 proximal one end of the connector 3580. The spring3520 provides an axial loading force which biases the washer 3525 andtherefore the sleeve 3510 and tubing 3505, as well as the tip 3515,towards the holder 3560. The spring 3520 may be selected to provide apreselected force adapted to be sufficient to force the tip 3515 againstone side of the holder 3560 as shown in FIG. 35 to provide a sealingengagement which does not leak and which will be sufficient to preventthe tubing 3505 from being forced out of a sealing engagement due to theintended pressures for the fluid flowing through the tubing 3505. Theholder 3560 has a tapered or frusto-conical portion 3561 to provide areduced surface area against which the tip 3515 provides a sealingengagement. This allows the assembly 3501 to handle a greater fluidpressure without requiring an operator to significantly add a greateraxial force to obtain a sealing engagement. As noted above, the tubing3505, holder 3560, tip 3515, filter 3562, and/or the column 3510 maycomprise biocompatible materials for applications in whichbiocompatibility is desired. In addition, these items may comprisematerials selected for a particular intended application, such as thoseinvolving higher or lower pressures, or involving high or low pH levels,or those involving particular fluids, such as may be corrosive.

Those skilled in the art will appreciate that the embodiments shown anddescribed in the present disclosure can be used in a variety ofdifferent configurations. For example, although the embodiments shownand described above have referred to use with flat-bottomed ports, thoseskilled in the art will appreciate that the assembly in its variousembodiments of this disclosure can also be used in coned ports or othertypes of connections. In addition, various types of tubing and fittingassembly configurations may be used in addition to those shown anddescribed, including without limitation those face-sealing assembliesshown and described in co-pending U.S. patent application Ser. No.14/922,041 filed on Oct. 23, 2015, and titled “Face-Sealing FluidicConnection System,” which was published as U.S. published patentapplication US 2016/0116088 A1 on Apr. 28, 2016, and is herebyincorporated by reference as fully set forth herein.

In addition to the tubing assemblies shown and described in US PublishedPatent Application No. 2016/0116088 A1, other types of tubing assembliesmay be used in accordance with various embodiments of the presentdisclosure. Turning to FIG. 36, for example, a tubing assembly 3601 isshown in a partial cross section. The assembly 3601 includes a tube3605, a sleeve 3610, and a tip 3615. It can be seen from FIG. 36 thatthe tip 3615 has a first end with a reduced surface area 3620. Thereduced surface area 3620 extends outward and defines a first end of thetip 3615, so that when the tubing assembly 3601 is inserted into a portor other connection assembly member to form a sealing connection, thereduced surface area 3620 is the portion of the tip 3615 which abuts thebottom of a port. By having reduced surface area 3620 in sealingengagement, the stresses are concentrated in a smaller area (i.e., thereduced surface area 3620 instead of over an area defined by the outerdiameter of the tip 3615), thus allowing for a sealing connection whichcan withstand higher pressures of fluid flow through the tubing assembly3601.

The tube 3605 can be any one of a number of materials. In one particularembodiment, tube 3604 may be a capillary tube. Similarly, sleeve 3610can be comprised of various materials. Tip 3615 also can be made ofvarious materials. The tubing 3605, tip 3615, and sleeve 3610 maycomprise any one or more of the following materials, and need notcomprise the same material as each other. Tubing 3605, sleeve 3610, andtip 3615 may comprise any one or more of materials, which may includemetal, such as steel, including stainless steel, aluminum, titanium, aswell as polymeric materials such as polyetheretherketone (PEEK),polyoxymethylene (POM) such as available under the mark DELRIN and knownsometimes as acetal, RADEL brand polyphenylsulfone (PPSU), ULTEM brandpolyetherimide (PEI), polyetherketoneketone (PEKK), polyaryletherketone(PAEK), polyethylene, polypropylene, polyvinylchloride, acrylic, and/orother materials, such a fused silica, silica borite, PEEKsil, and thelike.

Referring now to FIG. 37, further details of the end of the tubingassembly 3601 can be seen. As shown in FIG. 37, one end of the tubingassembly 3601 may include an end of a capillary tube 3605 which has apassageway 3603 extending therethrough for fluid flow. The end of thetube 3605 fits within a seating portion of the tip 3615. As shown inFIG. 37, tip 3615 has one end which covers and surrounds the outersurface of a portion of the tube 3605. In addition, the tip 3615 has asecond end which has a reduced surface area portion 3620, and the secondend of the tip 3615 is located between the end face of the tube 3605 anda port bottom surface (not shown in FIG. 37). As also shown in FIG. 37,the reduced surface area portion 3620 of the tip 3615 has a smallerdiameter than the outer diameter of the tip 3615, and extends outwardlyfrom the shoulder portions 3622 and 3624 of the tip 3615. Tip 3615 alsohas a passageway 3618 extending therethrough, which is preferablyaligned with the fluid passageway 3603 of the tube 3605.

Referring now to FIGS. 38A, 38B, and 38C, additional views of the tip3615 are provided. FIG. 38B is a side view of the tip 3615, with thereduced surface area or boss 3620 shown on the left side. FIG. 38Aprovides a top view of the tip 3615, and also shows the boss 3620 andthe fluid pathway 3618. FIG. 38C is a sectional view of the tip 3615taken along line A-A of FIG. 38A, and shows the reduced surface area3620, the shoulder portion 3622 (which is shown as curved in FIG. 38C,but could be angled or even be provided with a right angle if desired).In addition, FIG. 38C shows the passageway 3618 through the end of thetip 3615 and also the hollow seating portion 3628, which is adapted toreceive the end of a tube therein, such as is shown in FIG. 37.

Referring now to FIGS. 39-41, an alternative embodiment of a quickconnect/disconnect assembly 4001 in accordance with the presentdisclosure is shown, together an embodiment of the quickconnect/disconnect assembly 4001 with an adapter 4050 and a union 4070to form a further assembly. In FIG. 39, the assembly 4001 is shown in across-sectional view in an assembled state. The assembly 4001 includes abody 4002, tubing 4005, a spring 4020, a cap 4006, and a latch 4008. Asshown in FIG. 39, the tubing 5 extends through each of the body 4002,tubing 4005, spring 4020, cap 4006, and latch 4008, and extends from anopening on the left side of the assembly 4001. At one of end of thetubing 4005, a tip 4015 is provided. As also shown in FIG. 39, a portionof the tip 4015 and the tubing 4005 are enclosed by a sleeve 4010. Inaddition, it can be seen that the spring 4020 is located within a hollowportion of the body 4002, and one end of the spring 4020 abuts aninterior surface of one end of the body 4002. In addition, a second endof the body 4002 extends into an opening in the first end of the cap4006 and into an opening in one end of the latch 4008.

In FIG. 40, an exploded cross-sectional view of the assembly 4001 isprovided. For convenience of the reader, like numerals are used in FIGS.39-41 for like features. As shown in FIG. 40, the body 4002 is generallyhollow, with one end having an opening therethrough for the tubing 4005and the other end having an opening adapted to receive the spring 4020.The hollow portion of the body 4002 is adapted to receive and hold allor a portion of the spring 4020. As shown in FIGS. 39-41, the spring4020 is a coiled spring, and can be made of a suitable metal. Thoseskilled in the art will appreciate, however, especially from theforegoing disclosure, that the spring 4020 may be any one of the varioustypes of springs described above and made be made of any of thepotential materials noted earlier with respect to the springs describedabove.

A washer 4025 is also shown in FIG. 40. The washer 4025 abuts againstone end of the spring 4020, while the other end of the spring 4020 abutsan interior surface of one end of the body 4002. The relationship of thetube 4005, sleeve 4010, and tip 4015 can be as described above for suchan assembly, and the tube 4005, sleeve 4010, and tip 4015 can be of anyof the shapes and made of any of the materials noted above with respectto the earlier description thereof.

The cap 4006 shown in FIG. 40 has opposing openings 4012 a and 4012 b atopposing ends thereof. In addition, the cap 4006 has an opening 4012 cwhich extends into a hollow interior portion adapted to receive and holdthe latch 4008. In addition, the cap 4006 has a pocket 4012 d which isopen to the hollow interior portion 4012 c. The pocket 4012 d is adaptedto receive and hold a second spring 4007. As shown in FIG. 40, thespring 4007 is a coiled tapered spring. Those skilled in the art willappreciate, however, that the spring 4007 may be any one of the types ofsprings described earlier in this disclosure.

Still referring to FIG. 40, the latch 4008 is shown. The latch 4008 hasa central passageway therethrough that is adapted to allow the tubing4005, sleeve 4010, and tip 4015 combination (or just one or morecomponents thereof) to fit through the latch 4008. In addition, thelatch 4008 includes a radially inwardly extending projection 4008 a. Theradially inwardly projection 4008 a is adapted to cooperate with one endof an adapter 4050 like that shown in FIG. 41.

In FIG. 41, the assembly 4001 is shown with the tubing 4005 extendingtherethrough. In addition, an adapter 4050 and a union 4070 are shown.The adapter 4050 has a first end 4057 and a second end 4052. The firstend 4057 is adapted to fit into and be held securely by the openings4012 in cap 4006 and the interior portion 4014 of the latch 4008. Asshown in FIG. 41, the first end 4057 has radially outward projections4053, as well as an annular notch or ring 4055 with a smaller outsidediameter than the annular projection 4057. In addition, the projection4057 has a ramped portion facing towards the opening 4012. The rampedportion is adapted to cooperate with a ramped portion of the projection4008 a of the latch 4008. The ramped portions are adapted so that notools or excessive force is required to insert the first end 4057 of theadapter 4050 into the opening 4012 and engage the ramped portions sothat the projection 4008 a fits into at least a portion of the annularnotch 4055 and thereby securely holds the adapter 4050 and the cap 4006(and thereby the assembly 4001) together. In addition, the adapter 4050includes a second annular flange which extends radially outwardly in acentral portion of the adapter 4050. Near the second end 4052 of theadapter 4050 are external threads 4054.

As shown in FIG. 41, the external threaded portion 4054 of the adapter4050 is adapted to removably engage with the internally threaded portion4074 of a first end of the union 4070. The first end of the union 4070further has a flat-bottomed port 4072 for sealing engagement with thetip 4015 when the tip 4015 is pressed against the bottom of the port4072. Those skilled in the art will appreciate that, although a union4070 is shown in FIG. 41, any one of a number of different components ofan analytical instrument system may comprise a port that may beconnected as described to the assembly 4001 and adapter 405 instead ofthe union 4070. For example, other components which might have portssealingly connected to the assembly 4001 and adapter 4050 instead of theunion 4070 include at least the following: a column, or may be a portwhich is part of a manifold, a pump, a valve, a column, a filter, aguard column, a detector, a pressure regulator, a reservoir, a degasser,a debubbler, a union, a tee, a cross, a splitter, a sample loop, aconnector, or another component in an AI instrument or system. The meansfor exerting an axial force may comprises a spring, including withoutlimitation a coiled spring.

The fluidic connection assembly 4001, as well as adapter 4050 and union4070, in accordance with the present disclosure may comprise any one ormore of various materials, which may include metal, such as steel,including stainless steel, aluminum, titanium, as well as polymericmaterials such as polyetheretherketone (PEEK), polyoxymethylene (POM)such as available under the mark DELRIN and known sometimes as acetal,RADEL brand polyphenylsulfone (PPSU), ULTEM brand polyetherimide (PEI),polyetherketoneketone (PEKK), polyaryletherketone (PAEK), polyethylene,polypropylene, polyvinylchloride, acrylic, and/or other materials, sucha fused silica, silica borite, PEEKsil, and the like. The tubing 4005,tip 4015, and sleeve in the assembly 4001 similarly may comprise any oneor more of the foregoing materials. In addition, those skilled in theart will appreciate that the various constituent components of theassembly 4001, and/or adapter 4050 and/or union 4070, may comprise thesame or may comprise different materials. For example, assembly 4001 inone particular embodiment may have a body 4002 which comprises PEEK, acap 4006 which comprises PEEK, and a latch 4008 which comprises PEEK,while springs 4020 and 4007 comprise a metal such as stainless steel,and such an assembly 4001 can be used with an adapter 4050 made of metalsuch as stainless steel, and a union 4070 which comprise a metal orPEEK. Those skilled in the art will further appreciate that, for thosecomponents made of polymeric materials, the polymeric material mayinclude fibers, including carbon fibers, metallic fibers, and the like.It will be further appreciated that, even if an adapter 4050 comprisesmetal, the overall fluidic connection made with assembly 4001 can stillbe biocompatible, as long as the tubing tip 4015 of the tubing 4005extending through the assembly 4001, the adapter 4050, and in sealingengagement with the bottom of the port 4072 of the union 4070, as wellas the union 4070, comprise biocompatible materials.

Those skilled in the art will also appreciate that the material and sizeof spring members 4020 and 4007 can be selected so that the springmembers 4020 and 4007 provide the desired amount of force, or biasing,when engaged (as described in more detail above) in a fluidic connectionin a desired application. For example, one of skill would appreciatethat it would be advantageous to have the spring member 4020 and spring4007 provide a greater biasing force when the assembly 4001 will be usedin an application with a greater fluidic pressure, than might be thecase if the assembly 4001 is to be used in an application with arelatively small fluidic pressure. For example, not all fluidicconnections in a given AI system used for a given application needoperate under the same fluid pressure. The fluid pressures may changeeven in such situations for different connections in the AI system.

Methods of making a fluidic connection or disconnecting tubing from aport or component with respect to the assembly 4001 as shown in FIGS.39-41 are now summarized. In making a connection, an operator or usermay perform the steps of providing a tube 4005 having first and secondends, wherein the second end may comprise a compressible tip, insertingone end of the tube through a body 4002, wherein said body 4002comprises a first end and a second end, said body 4002 further having apassageway therethrough adapted to receive a tube 4005 extending throughsaid body, and wherein an interior portion proximal the second end ofsaid body is adapted to cooperate with a spring 4020 for exerting aforce against a washer or disc 4025 which in turn exerts a force againsta sleeve 4010 which surrounds a portion of the tube 4005, thus biasingthe end of the tube 4005 in a longitudinal direction away from the body4002. In addition, the operator or user may insert a portion of a latch4008 into a portion of a cap 4006 adapted to receive the latch 4008portion. A spring 4007 may also be inserted into a recess in the cap4006, with the spring 4007 adapted to urge the latch upward and radiallyoutwardly. The user or operator alternatively may simply obtain a tube4005 and place one end through the interior passageway of the assembly4001 once assembled. In either case, an adapter 4050 can be provided andthe user or operator can insert one end of the tube 4005 into thepassageway through the adapter 4050, and insert one end 4057 of theadapter 4050 into the opening 4012 of the cap and the interior portionsof latch 4008. As noted, the annular projection or flange 4053 of theadapter has ramped portions adapted to engage with the ramped portionsof the latch 4008 a to allow the user or operator to compress the spring4020 more easily and have the latch 4008 securely engage with and holdthe adapter 4050 in place. An operator or user can then insert thethreaded end of the adapter 4050 into a port or other component such asany of the types described above, and then engage the threaded portionof the port or component (such as the union 4070) to obtain a secure andsealed fluidic connection. To disconnect the assembly 4001 from the portor component, a user may turn the assembly 4001 and/or the port or othercomponent (such as union 4070) in the opposite direction to disengagethe threaded portions from one another, then remove the adapter 4050 andassembly 4001 and the tube 4005 from the port or other component.

The spring 4020 may be selected to provide a preselected force or rangeof force adapted to be sufficient to force the tip 4015 against thebottom face of the port or component to provide a sealing engagementwhich does not leak and which will be sufficient to prevent the tubing4005 from being forced out of a sealing engagement due to the intendedpressures for the fluid flowing through the tubing 4005. It will also beappreciated that the spring 4020 may be selected so that the forcerequired to compress the spring, such as by inserting one end of theadapter 4050 into the cap 4006 and latch 4008 and securely engage theadapter 4050 with the assembly 4001, is relatively limited and does notrequire any tools or equipment, but can be done by an operator or userby hand. Moreover, it will be appreciated that the adapter 4050 andassembly 4001 connection, once made, may be permanent or may be aremovable connection, especially if the intended use of the assembly4001 is for lower pressure applications and therefore the force requiredto keep the assembly 4001 and adapter 4050 secured together is not asgreat. In such situations, the second spring 4007 can be selected toprovide a relatively lower force against the bottom of the latch 4008.

Those skilled in the art will also appreciate that the embodiments shownand described in the present disclosure can be used in a variety ofdifferent configurations. For example, although the embodiments shownand described above have referred to use with flat-bottomed ports, thoseskilled in the art will appreciate that the assembly in its variousembodiments of this disclosure can also be used in coned ports or othertypes of connections, including those described above. In addition,various types of tubing and fitting assembly configurations may be usedin addition to those shown and described, including without limitationthose face-sealing assemblies shown and described in co-pending U.S.patent application Ser. No. 14/922,041 filed on Oct. 23, 2015, andtitled “Face-Sealing Fluidic Connection System,” which was published asU.S. published patent application US 2016/0116088 A1 on Apr. 28, 2016,and is hereby incorporated by reference as fully set forth herein.

In addition to the tubing assemblies shown and described in US PublishedPatent Application No. 2016/0116088 A1, other types of tubing assembliesmay be used in accordance with various embodiments of the presentdisclosure, such as those illustrated in FIGS. 36-38 and describedabove, for example. The tube 4005 can be any one of a number ofmaterials. In one particular embodiment, tube 4005 may be a capillarytube. Similarly, sleeve 4010 can be comprised of various materials. Tip4015 also can be made of various materials. The tubing 4005, tip 4015,and sleeve 4010 may comprise any one or more of the following materials,and need not comprise the same material as each other, and suchmaterials may include metal, such as steel, including stainless steel,aluminum, titanium, as well as polymeric materials such aspolyetheretherketone (PEEK), polyoxymethylene (POM) such as availableunder the mark DELRIN and known sometimes as acetal, RADEL brandpolyphenylsulfone (PPSU), ULTEM brand polyetherimide (PEI),polyetherketoneketone (PEKK), polyaryletherketone (PAEK), polyethylene,polypropylene, polyvinylchloride, acrylic, and/or other materials, sucha fused silica, silica borite, PEEKsil, and the like.

We believe that the assembly 4001 can be used to achieve leak-free,sealing connections without any extrusion of tubing 4005 from a port(such as port 4072) when fluid is flowing through the tubing 4005 atpressures of up to at least 20,000 psi, including for biocompatibleapplications.

Referring now to FIGS. 42-46, an alternative embodiment of the quickconnect/disconnect assembly 5001 in accordance with the presentdisclosure is shown, together with an embodiment of the quickconnect/disconnect assembly 5001 with an adapter 5050 to form thefurther assembly 5001 a. For convenience of the reader, like numeralsare used in FIGS. 42-46 for like features. In FIG. 42, the assembly 5001is shown in an exploded three-dimensional view. The assembly 5001includes a body 5002, a spring 5020, a cap 5006, and a latch 5008.Although not shown in FIG. 42, those skilled in the art will appreciatethat tubing (not shown in FIG. 42) can extend through each of the body5002, spring 5020, cap 5006, and latch 5008, and can extend from anopening on the left side of the assembly 5001, as well as the right sideof the assembly 5001.

The body 5002 is generally hollow, with one end (the right hand side asshown in the orientation of FIG. 42) having an opening therethroughadapted to receive tubing (not shown in FIG. 42) and the other end (theleft hand side in the orientation shown in FIG. 42) having an openingadapted to receive the spring 5020. The hollow portion of the body 5002is adapted to receive and hold all or a portion of the spring 5020. Asshown in FIG. 42, the spring 5020 is a coiled spring, and can be made ofany suitable metal. Those skilled in the art will appreciate, however,especially from the foregoing disclosure, that the spring 5020 may beany one of the various types of springs described above and made be madeof any of the potential materials noted earlier with respect to thesprings described above. In addition, the body 5002 has a pocket 5002 dwhich is open to the body's hollow interior portion. The pocket 5002 dis adapted to receive and hold the second spring 5007. As shown in FIG.42, the spring 5007 is a coiled tapered spring. Those skilled in the artwill appreciate, however, that the spring 5007 may be any one of thetypes of springs described earlier in this disclosure.

A washer 5025 is also shown in FIG. 42. The washer 5025 abuts againstone end of the spring 5020, while the other end of the spring 5020 abutsan interior surface of one end of the body 5002. The cap 5006 shown inFIG. 42 has an opening 5012. In addition, the cap 5006 has an extension5011 on one side thereof, and the extension 5011 has a latching orholding member 5011 a, which projects radially inward towards thelongitudinal axis of the assembly 5001.

Still referring to FIG. 42, the latch 5008 is shown. The latch 5008 hasa central passageway therethrough. In addition, the latch 5008 includesa radially inwardly extending projection 5008 a. The radially inwardlyextending projection 5008 a is adapted to cooperate with one end of anadapter 5050 like that shown in FIG. 43, as explained in more detailbelow. The latch 5008 on its top portion in the orientation shown inFIG. 42 further has opposing projections 5009 a and 5009 b. As shown inFIG. 42, the top of the latch 5008 is angled or arcuate, so that thethickness of the latch 5008 at the outer sides of projections 5009 a and5009 b is relatively thinner than towards the center of the top of thelatch 5008. It will be appreciated that the top of the latch 5008,including projections 5009 a and 5009 b, is sized and adapted to slideinto (and out of, if desired) the corresponding top right portion of thebody 5002. The body 5002 further includes recesses 5003 a and 5003 bwhich are adapted to removably receive and securely hold the projections5009 a and 5009 b, respectively, of the latch 5008. When assembled, theprojections 5009 a and 5009 b fit into and are securely held in therecesses 5003 a and 5003 b.

Now referring to FIG. 43, the assembly 5001 is shown with tubing 5005extending therethrough. In addition, an adapter 5050 is shown. Theadapter 5050 has a first end and a second end. The first end of theadapter 5050 is adapted to fit into and be held securely in place by theinterior extending projection 5008 a of the latch 5008. As shown in FIG.43, the first end has a radially outward projection 5053, as well as anannular notch or ring 5055 with a smaller outside diameter than theannular projection 5053. In addition, the projection 5053 has a rampedportion facing towards the opening 5012. The ramped portion of theannular flange 5053 is adapted to cooperate with a ramped portion of theprojection 5008 a of the latch 5008. The ramped portions are adapted sothat no tools or excessive force is required for an operator or user toinsert the first end 5053 of the adapter 5050 into the opening 5012 andengage the ramped portions so that the projection 5008 a fits into atleast a portion of the annular notch 5055 and thereby securely holds theadapter 5050 and the latch 5008 (and thereby the assembly 5001)together. In addition, the adapter 5050 includes a second annular flange5051 which extends radially outwardly in a central portion of theadapter 5050. Near the second end 5052 of the adapter 5050 are externalthreads 5054.

As also shown in FIG. 43, one end of the tubing 5005 has a tip 5015provided. As also shown in FIG. 43, at least a portion of the tip 5015and the tubing 5005 are enclosed by a sleeve 5010. The relationship ofthe tube 5005, sleeve 5010, and tip 5015 can be as described above, andthe tube 5005, sleeve 5010, and tip 5015 can be of any of the shapes andmade of any of the materials noted above with respect to the earlierdescription thereof. Those skilled in the art will further appreciatethat other types of tubing, such as without tip 5015 and/or without asleeve 5010, or with different configurations for either or both the tip5015 and/or sleeve 5010, can be used with the assembly 5001 as describedherein.

In FIG. 44, assembly 5001 a is shown in an assembled state. The assembly5001 a includes the body 5002, tubing 5005, the latch 5008, the cap5006, the adapter 5050, and the tip 5015. As can be seen from acomparison of FIGS. 43 and 44, FIG. 44 shows the adapter 5050 asinserted into the opening of the cap 5006 and held by the latch 5008projection 5008 a (not seen in FIG. 44). As assembled, the adapter 5050,cap 5006, latch 5008, and body 5002 form an assembly 5001 a which willsecurely hold together, and which can be used to secure one end of thetubing 5005 (or, as shown in FIG. 44, the tip 5015 at one end of thetubing 5005) to a port of or to another component in a fluidic system,such as an analytical instrument system.

Referring now to FIG. 45, a cross-sectional view of the assembly 5001and the adapter 5050 is provided. As can be seen, the adapter 5050 has afirst end 5057 and a second end 5052. Proximal the first end 5057 is anannular flange or radially outwardly extending projection 5053. Theramped portion of the flange 5053 is shown and is located on the side ofthe flange 5053 facing the assembly 5001. In addition, a portion of theadapter behind the flange 5053 has a smaller diameter and can beconsidered a notch portion 5055 directly behind or proximal behind theflange 5053. In addition, the adapter has external threads 5054 and asecond end adapted to fit into and form a seal in a flat bottomed portor other component.

Still referring to FIG. 45, it can be seen that the spring 5020 islocated within a hollow portion of the body 5002, and one end of thespring 5020 abuts an interior surface of one end of the body 5002. Inaddition, a second end of the body 5002 is removably attached to oneside of the latch 5008. As shown in FIG. 45, the second end of the body5002 provides a recess in which the second spring 5007 is located. Aswill be appreciated by those skilled in the art, the second spring 5007is used to press the inward projection 5008 a of the latch 5008 into thenotch 5055 of the adapter 5050 when the first end 5057 of the adapter5050 is inserted into the second end of the assembly 5001. Similarly, itwill be appreciated that the spring 5020 is adapted to press the washer5025 and, in turn, one end of the sleeve 5010 and also the tip 5015,away from the second end of the body 5002 (and towards the left side ofthe view shown in FIG. 45 in this orientation of the assembly 5001).

It will be appreciated that the external threaded portion 5054 of theadapter 5050 is adapted to removably engage with an internally threadedportion of a port or other component (not shown in FIG. 45). In theparticular configuration of the adapter 5050 as shown, it will beappreciated the end 5052 of the adapter 5050 is adapted for use with aflat-bottomed port for sealing engagement with the tip 5015 when the tip5015 is pressed against the bottom of the port. Those skilled in the artwill appreciate that, although a flat-bottomed port has been described,any one of a number of different components of an analytical instrumentsystem may comprise a port that may be connected as described to theassembly 5001 and adapter 5050. For example, other components whichmight have ports sealingly connected to the assembly 5001 and adapter5050 include at least the following: a column, or may be a port which ispart of a manifold, a pump, a valve, a column, a filter, a guard column,a detector, a pressure regulator, a reservoir, a degasser, a debubbler,a union, a tee, a cross, a splitter, a sample loop, a connector, oranother component in an AI instrument or system. The means for exertingan axial force may comprises a spring, including without limitation acoiled spring. In addition, those skilled in the art will appreciatethat, although not shown, the end 5052 of the adapter 5050 may be of aconfiguration that is adapted to provide a sealing engagement in afrusto-conical shaped port or component.

FIG. 46 provides a cross-sectional view of the assembly 5001 a asassembled and ready for insertion into a flat-bottomed port or componentof an analytical instrument system (not shown) to provide a sealingengagement.

It will be appreciated from the foregoing disclosure that the assembly5001 a allows an operator or user to easily insert the end of the tubinghaving the tip 5015 and the end 5052 of the adapter 5050 into aflat-bottomed port by engaging internal threads of the port or component(not shown) with the external threads of the adapter 5050. The forceexerted by the biasing of the spring 5020 will maintain a sealingengagement of the tip 5015 and the bottom face of the port of component.We believe that the assembly 5001 a can be used to achieve leak-free,sealing connections without any extrusion of tubing 5005 from a portwhen fluid is flowing through the tubing 5005 at pressures of up to atleast 20,000 psi, including for biocompatible applications.

The fluidic connection assembly 5001 and assembly 5001 a, as well asadapter 5050, in accordance with the present disclosure may comprise anyone or more of various materials, which may include metal, such assteel, including stainless steel, aluminum, titanium, as well aspolymeric materials such as polyetheretherketone (PEEK),polyoxymethylene (POM) such as available under the mark DELRIN and knownsometimes as acetal, RADEL brand polyphenylsulfone (PPSU), ULTEM brandpolyetherimide (PEI), polyetherketoneketone (PEKK), polyaryletherketone(PAEK), polyethylene, polypropylene, polyvinylchloride, acrylic, and/orother materials, such a fused silica, silica borite, PEEKsil, and thelike. The tubing 5005, tip 5015, and sleeve 5010 in the assembly 5001similarly may comprise any one or more of the foregoing materials. Inaddition, those skilled in the art will appreciate that the variousconstituent components of the assembly 5001, and/or adapter 5050, maycomprise the same or may comprise different materials. For example,assembly 5001 in one particular embodiment may have a body 5002 whichcomprises PEEK, a cap 5006 which comprises PEEK, and a latch 5008 whichcomprises PEEK, while springs 5020 and 5007 comprise a metal such asstainless steel, and such an assembly 5001 can be used with an adapter5050 made of metal such as stainless steel. Those skilled in the artwill further appreciate that, for those components made of polymericmaterials, the polymeric material may include fibers, including carbonfibers, metallic fibers, and the like. It will be further appreciatedthat, even if an adapter 5050 comprises metal, the overall fluidicconnection made with assembly 5001 a can still be biocompatible, as longas the tubing tip 5015 of the tubing 5005 extending through the assembly5001 a, and in sealing engagement with the bottom of the port, comprisebiocompatible materials.

Those skilled in the art will also appreciate that the material and sizeof spring members 5020 and 5007 can be selected so that the springmembers 5020 and 5007 provide the desired amount of force, or biasing,when engaged (as described in more detail above) in a fluidic connectionin a desired application. For example, one of skill would appreciatethat it would be advantageous to have the spring member 5020 and spring5007 provide a greater biasing force when the assembly 5001 will be usedin an application with a greater fluidic pressure, than might be thecase if the assembly 5001 is to be used in an application with arelatively small fluidic pressure. For example, not all fluidicconnections in a given AI system used for a given application needoperate under the same fluid pressure. The fluid pressures may changeeven in such situations for different connections in the AI system.

Methods of making a fluidic connection or disconnecting tubing from aport or component with respect to the assembly 5001 and FIGS. 42-46 arenow summarized. In making a connection, an operator or user may performthe steps of providing a tube 5005 having first and second ends, whereinthe second end may comprise a compressible tip, inserting one end of thetube through a body 5002, wherein said body 5002 comprises a first endand a second end, said body 5002 further having a passagewaytherethrough adapted to receive a tube 5005 extending through said body,and wherein an interior portion proximal the second end of said body isadapted to cooperate with a spring 5020 for exerting a force against awasher or disc 5025 which in turn exerts a force against a sleeve 5010which surrounds a portion of the tube 5005, thus biasing the end of thetube 5005 in a longitudinal direction away from the body 5002. Inaddition, the operator or user may insert a portion (such as projections5009 a and 5009 b) of a latch 5008 into a portion of the body 5002adapted to receive and hold them, and insert a portion of a cap 5006into or around an exterior portion of the latch 5008 (such as projection5011 a and 5011, which are adapted to engage with a portion of latch5008 and hold cap 5006 and latch 5008 together). A spring 5007 may alsobe inserted into a recess in the body 5002, with the spring 5007 adaptedto urge the latch 5008 upward and radially outwardly.

The user or operator alternatively may simply obtain a tube 5005 andplace one end through the interior passageway of the assembly 5001 onceassembled. In either case, an adapter 5050 can be provided and the useror operator can insert one end of the tube 5005 into the passagewaythrough the adapter 5050, and insert one end 5057 of the adapter 5050into the opening of the cap 5006 and the interior portions of latch 5008and/or body 5002. As noted and described above, the annular projectionor flange 5053 of the adapter 5050 has ramped or angled portions adaptedto engage with the ramped portions of the latch 5008 to allow the useror operator to compress the spring 5020 more easily and have the latch5008 securely engage with and hold the adapter 5050 in place. Anoperator or user can then insert the threaded end of the adapter 5050into a port or other component such as any of the types described above,and then engage the threaded portion of the port or component (notshown) to obtain a secure and sealed fluidic connection. To disconnectthe assembly 5001 from the port or component, a user may turn theassembly 5001 and/or the port or other component (not shown) in theopposite direction to disengage the threaded portions from one another,then remove the adapter 5050 and assembly 5001 and the tube 5005 fromthe port or other component.

The spring 5020 may be selected to provide a preselected force or rangeof force adapted to be sufficient to force the tip 5015 against thebottom face of the port or component to provide a sealing engagementwhich does not leak and which will be sufficient to prevent the tubing5005 from being forced out of a sealing engagement due to the intendedpressures for the fluid flowing through the tubing 5005. It will also beappreciated that the spring 5020 may be selected so that the forcerequired to compress the spring, such as by inserting one end of theadapter 5050 into the cap 5006 and latch 5008 and securely engage theadapter 5050 with the assembly 5001, is relatively limited and does notrequire any tools or equipment, but can be done by an operator or userby hand. Moreover, it will be appreciated that the adapter 5050 andassembly 5001 connection, once made, may be permanent or may be aremovable connection, especially if the intended use of the assembly5001 is for lower pressure applications and therefore the force requiredto keep the assembly 5001 and adapter 5050 secured together is not asgreat. In such situations, the second spring 5007 can be selected toprovide a relatively lower force against the bottom of the latch 5008.

Those skilled in the art will also appreciate that the embodiments shownand described in the present disclosure can be used in a variety ofdifferent configurations. For example, although the embodiments shownand described above have referred to use with flat-bottomed ports, thoseskilled in the art will appreciate that the assembly in its variousembodiments of this disclosure can also be used in coned ports or othertypes of connections, including those described above. In addition,various types of tubing and fitting assembly configurations may be usedin addition to those shown and described, including without limitationthose face-sealing assemblies shown and described in co-pending U.S.patent application Ser. No. 14/922,041 filed on Oct. 23, 2015, andtitled “Face-Sealing Fluidic Connection System,” which was published asU.S. published patent application US 2016/0116088 A1 on Apr. 28, 2016,and is hereby incorporated by reference as fully set forth herein.

In addition to the tubing assemblies shown and described in US PublishedPatent Application No. 2016/0116088 A1, other types of tubing assembliesmay be used in accordance with various embodiments of the presentdisclosure, such as those illustrated in FIGS. 42-46 and describedabove, for example. The tube 5005 can be any one of a number ofmaterials. In one particular embodiment, tube 5005 may be a capillarytube. Similarly, sleeve 5010 can be comprised of various materials. Tip5015 also can be made of various materials. The tubing 5005, tip 5015,and sleeve 5010 may comprise any one or more of the following materials,and need not comprise the same material as each other, and suchmaterials may include metal, such as steel, including stainless steel,aluminum, titanium, as well as polymeric materials such aspolyetheretherketone (PEEK), polyoxymethylene (POM) such as availableunder the mark DELRIN and known sometimes as acetal, RADEL brandpolyphenylsulfone (PPSU), ULTEM brand polyetherimide (PEI),polyetherketoneketone (PEKK), polyaryletherketone (PAEK), polyethylene,polypropylene, polyvinylchloride, acrylic, and/or other materials, sucha fused silica, silica borite, PEEKsil, and the like.

While the present invention has been shown and described in variousembodiments, those skilled in the art will appreciate from the drawingsand the foregoing discussion that various changes, modifications, andvariations may be made without departing from the spirit and scope ofthe invention as set forth in the claims. Those skilled in the art willfurther appreciate that various materials can be used in place of or inaddition to those described herein, and that the embodiments shown anddescribed can be used in additional applications and provide additionaladvantages beyond those set forth herein. Hence, the embodiments shownand described in the drawings and the above discussion are merelyillustrative and do not limit the scope of the invention as defined inthe claims herein. The embodiments and specific forms, materials, andthe like are merely illustrative and do not limit the scope of theinvention or the claims herein.

We claim:
 1. A fluidic connection assembly comprising: a body having afirst end and a second end, each of the first and second ends havingopenings therein, and having a hollow portion therein; a first springlocated at least partially within the hollow portion of said body, andhaving a first end abutting an interior surface of the hollow portion ofsaid body; a latching member having an opening therethrough and having afirst portion adapted to fit at least partially within said body; a capmember having an opening therethrough and having first and second sides,wherein a portion of the first side of said cap member is adapted toextend into at least a portion of the hollow portion of said body andwherein the second side of said cap member is adapted to be attached tothe second end of said body; a tube having a first outer diameter andextending through the body, the first spring, and the cap member,wherein the tube has an end that extends outwardly from the cap memberand the body and has a sealing face having a second diameter that isless than the first outer diameter; and wherein said latching member isadapted to engage with and securely hold an adapter when a portion ofone end of the adapter is inserted into the opening of said cap memberand the opening of said latching member.
 2. The fluidic connectionassembly of claim 1 wherein said first spring is in a compressed statewhen said cap member, said latching member, and said body are assembledtogether and said first spring is located entirely within the hollowportion of said body.
 3. The fluidic connection assembly of claim 1further comprising a second spring, wherein said second spring islocated within the hollow portion of said body and adjacent to at leastone side of said latching member.
 4. The fluidic connection assembly ofclaim 3 wherein said second spring is adapted to bias said latchingmember so that a radially inward projection of said latching member ispressed against a portion of said adapter when a first end of theadapter is inserted into said latching member.
 5. The fluidic connectionassembly of claim 1 wherein said first spring exerts a force which urgesthe end of said tube away from said body.
 6. The fluidic connectionassembly of claim 5 further comprising a washer located within said bodyand adjacent to a second end of said first spring, a sleeve membersurrounding at least a portion of said tube, wherein said sleeve memberhas a first end and a second end, and wherein said first spring exerts aforce on said washer and said washer exerts a force on said sleevemember, and said sleeve member exerts a force on said tube which urgesthe one end of said tube away from said body.
 7. A fluidic connectionassembly comprising: a body having a first end and a second end, each ofthe first and second ends having openings therein, and having a hollowportion therein; a first spring member located at least partially withinthe hollow portion of said body; a latch member having an openingtherethrough and having a base portion adapted to removably fit at leastpartially within said body; a cap member having an opening therethroughand having first and second sides and is adapted to removably hold saidlatch member and said cap member together and to be attached to thesecond end of said body, and wherein said latch member is adapted toremovably hold an adapter when a portion of the adapter is inserted intothe opening of said cap member and the opening of said latch member,wherein each of said body, said first spring member, said latch member,and said cap member have a passage therethrough; and a tube having amaximum outer diameter and extending through the passage of the body,the first spring member, the latch member, and the cap member, whereinan end of the tube extending from the cap member has a sealing portionhaving a diameter that is less than the maximum outer diameter of thetube.
 8. The fluidic connection assembly of claim 7 wherein said firstspring member is in a compressed state when said cap member, said latchmember, and said body are assembled together and said first springmember is located entirely within the hollow portion of said body. 9.The fluidic connection assembly of claim 7 further comprising a secondspring member, wherein said second spring member is at least partiallylocated within a pocket portion of said body and adjacent to at leastone side of said latch member.
 10. The fluidic connection assembly ofclaim 9 wherein said second spring member is adapted to bias said latchmember so that a radially inward projection of said latch member ispressed against a portion of said adapter when the adapter is insertedinto said latch member.
 11. The fluidic connection assembly of claim 7wherein said first spring member urges the end of said tube away fromsaid body.
 12. The fluidic connection assembly of claim 7 furthercomprising a washer located within said body and adjacent to a secondend of said first spring member and a passageway comprising tubing and asleeve member surrounding at least a portion of said tubing, said sleevemember having a first end and a second end, and wherein said firstspring member exerts a force on said washer and said washer exerts aforce on said sleeve member, and said sleeve member urges an end of saidtubing away from said body.
 13. The fluidic connection assembly of claim7 wherein at least one of said body, said latch member, and said capmember comprises polyetherketoneketone.
 14. The fluid connectionassembly of claim 7, wherein said assembly is adapted to provide aleak-free connection of one end of tubing when fluid is flowing throughthe tubing at pressures up to at least 5,000 psi.
 15. The fluidicconnection assembly of claim 7, wherein when a first end of the adapteris located within the second end of the body, a radially inwardprojection of the latch member presses against a portion of the firstend of the adapter.
 16. The fluidic connection assembly of claim 15,wherein a second end of the adapter is adapted to removably engage aport.
 17. The fluidic connection assembly of claim 16 wherein the portcomprises a flat-bottomed port.
 18. A fluidic connection assemblycomprising: a body having a first end and a second end, each of thefirst and second ends having openings therein, and having a hollowportion therein; a first spring located within the hollow portion of thebody, and having a first end abutting an interior surface proximal thefirst end of the body; a latch member having an opening therethrough andadapted to be removably attached to a portion of the body; a cap memberhaving an opening therethrough and having first and second sides,wherein the second side of the cap member is adapted to be attached tothe second end of the body, and wherein the latch member is adapted toremovably hold an adapter when a portion of one end of the adapter isinserted into the opening of the cap member and the opening of the latchmember, wherein each of the body, the first spring, the latch member,and the cap member have a passage therethrough; and a tube extendingthrough the passages of the body, the first spring, the latch member,and the cap member and having a first end having a first portion havinga first outer diameter and a second portion having a second outerdiameter, wherein the second outer diameter is less than the first outerdiameter and the second portion is adapted to sealingly engage with aport.
 19. The fluidic connection assembly of claim 18 further comprisinga second spring, wherein said second spring is located within the hollowportion of the body and adjacent to at least one side of the latchmember.
 20. The fluidic connection assembly of claim 19 wherein thesecond spring is adapted to bias the latch member so that a radiallyinward projection of the latch member is pressed against a portion ofthe adapter when a first end of the adapter is inserted into the latchmember.
 21. A fluidic connection assembly comprising: a body having afirst end and a second end, each of the first and second ends havingopenings therein, and having a hollow portion therein; a first springlocated within the hollow portion of the body, and having a first endabutting an interior surface proximal the first end of the body; a latchmember having an opening therethrough and adapted to be removablyattached to a portion of the body; a cap member having an openingtherethrough, wherein each of the body, the first spring, the latchmember, and the cap member have a passage therethrough; and a tubeextending through the passages of the body, the first spring, the latchmember, and the cap member, and having a first end having a sealing facewith a first outer diameter and adapted to sealingly engage with aportion of a flat-bottomed port having a second outer diameter that isless than the first outer diameter of the tube.
 22. The fluidicconnection assembly according to claim 21, wherein the assembly isadapted to sealingly engage with the port without the application oftorque.
 23. The fluidic connection assembly according to claim 22,wherein the assembly is adapted to sealingly engage with the port byhand and without the use of a tool.
 24. The fluidic connection assemblyaccording to claim 23, wherein the assembly is adapted to sealinglyengage with the port when a fluid flows through the tube with a pressureof 5,000 psi or more.